Bhat P, Rajan D
Am J Clin Pathol. 1975 Sep;64(3):399-404. doi: 10.1093/ajcp/64.3.399.
Studies of the relative efficacies of different modes of transport of fecal specimens intended for culture for the isolation of Shigellae have been carried out. When the delay between collection and culture was long, transportation in liquid nitrogen was slightly but not significantly better than transportation on ice. When the delay was short (10 hours or less) transportation at ambient temperature was as satisfactory as that on ice. A comparative study of the efficacies of three media for the isolation of Shigellae was also carried out. A total of 75 stool specimens containing Shigellae was studied. Isolation rates were highest with xylose lysine desoxycholate medium (XLD) and desoxycholate citrate medium (DCA). XLD was superior to DCA in that the Shigella colonies were clearly visible after overnight incubation, whereas use of DCA necessitated 48 hours of incubation. Also, colonial morphology on XLD was more specific. It is recommended that both XLD and DCA be employed, but if only one medium is to be used, XLD is the medium of choice.
针对用于培养志贺氏菌的粪便标本的不同运输方式的相对有效性进行了研究。当采集与培养之间的延迟时间较长时，液氮运输略优于冰上运输，但差异不显著。当延迟时间较短（10小时或更短）时，常温运输与冰上运输效果相当。还对三种用于分离志贺氏菌的培养基的有效性进行了比较研究。共研究了75份含有志贺氏菌的粪便标本。木糖赖氨酸脱氧胆酸盐培养基（XLD）和柠檬酸盐脱氧胆酸盐培养基（DCA）的分离率最高。XLD优于DCA，因为志贺氏菌菌落经过一夜培养后清晰可见，而使用DCA则需要48小时培养。此外，XLD上的菌落形态更具特异性。建议同时使用XLD和DCA，但如果只使用一种培养基，XLD是首选。
