Reybrouck G, Borneff J, van de Voorde H, Werner H P
Zentralbl Bakteriol B. 1979 Jun;168(5-6):463-79.
In a collaborative study on a new quantitative suspension test for the evaluation of the bactericidal activity of chemical disinfectants, three laboratories performed the in vitro test on a phenol and an aldehyde standard in the critical use dilutions using Staphylococcus aureus and Pseudomonas aeruginosa as test organisms. The most striking finding was that the means of the germidical effect of one laboratory were significant lower than those of both others. Nevertheless the dispersion of the results did not differ among the laboratories. The differences could not be attributed to the subculture technique followed, nor to the daily inconstancy of the bacterial suspension resistance. The only feature that could explain the difference was the assessment of the microbiological work in itself. It should be stated, however, that the variance of the germicidal-effect values were rather low for this kind of microbiological work, so that differences between laboratories were significant even if the absolute values differed less than 1 unit.
在一项关于评估化学消毒剂杀菌活性的新型定量悬液试验的合作研究中，三个实验室使用金黄色葡萄球菌和铜绿假单胞菌作为试验微生物，对处于关键使用稀释度的一种酚类和一种醛类标准品进行了体外试验。最显著的发现是，一个实验室的杀菌效果均值明显低于其他两个实验室。然而，各实验室结果的离散度并无差异。这些差异既不能归因于所采用的传代培养技术，也不能归因于细菌悬液抗性的每日变化。唯一能够解释这种差异的因素是微生物学工作本身的评估。然而，应该指出的是，对于这类微生物学工作，杀菌效果值的方差相当低，因此即使绝对值差异小于1个单位，各实验室之间的差异也是显著的。
