Reybrouck G, van de Voorde H
Zentralbl Bakteriol Orig B. 1975 Aug;160(6):541-50.
The bactericidal activity of three disinfectant standards has been determined by four national methods (the qualitative suspension test of the German Society for Hygiene and Microbiology DGHM, the quantitative suspension test of the Dutch Committee on Phytopharmacy, the use-dilution method of the A.O.A.C. and the Kelsey and Sykes' test) in order to compare these four testing techniques. In previous publications the results of these experiments and the degree of standardisation of the four methods have been tested. The purpose of the present study is to evaluate to what extent the results obtained by the four testing techniques do correspond. The bactericidal activity can be expressed as a germicidal effect. In theory (Tab. 1) the values of germicidal effect can be similar for the four testing techniques. In practice (Tab. 2 and 3) it has been found that with the suspension technique of the DGHM systematically higher values are obtained than in the other tests. These can not be compared to each other, because the medication times differ. Although the values of the germicidal effect obtained are widely different, it can happen that the minimum effective concentration determined by the four methods be identical as each of these methods applies different criteria. The authors have therefore calculated the minimum effective concentration which is required for the disinfectant standard by the four disinfectant testing techniques (Tab. 4). Even here, no correspondence has been found between the four methods. The lethal concentration of the disinfectant standards determined by the use-dilution method of the A.O.A.C. is thus 2 to 16 times those required by the Dutch suspension test. The difference in effective concentrations, however, is not constant and is not related to the type of disinfectant or the test organism. In consequence, there is no way of establishing a comparison between the results of the four methods with a view to setting up a conversion table. Each disinfectant testing technique has its own characteristics.
为了比较四种检测技术，采用了四种国家标准方法（德国卫生与微生物学会DGHM的定性悬液试验、荷兰植物药剂委员会的定量悬液试验、美国分析化学家协会的使用稀释法以及凯尔西和赛克斯试验）测定了三种消毒剂标准品的杀菌活性。在以往的出版物中，对这些实验的结果以及这四种方法的标准化程度进行了检验。本研究的目的是评估这四种检测技术所获得的结果在多大程度上相符。杀菌活性可以表示为杀菌效果。理论上（表1），这四种检测技术的杀菌效果值可能相似。在实际操作中（表2和表3）发现，采用DGHM的悬液技术所获得的值系统性地高于其他试验。由于用药时间不同，这些值无法相互比较。尽管所获得的杀菌效果值差异很大，但由于每种方法应用的标准不同，四种方法测定的最低有效浓度可能相同。因此，作者计算了四种消毒剂检测技术测定消毒剂标准品所需的最低有效浓度（表4）。即使在此处，四种方法之间也未发现相符性。因此，美国分析化学家协会使用稀释法测定的消毒剂标准品的致死浓度是荷兰悬液试验所需浓度的2至16倍。然而，有效浓度的差异并不恒定，也与消毒剂类型或试验微生物无关。因此，无法为了建立换算表而对四种方法的结果进行比较。每种消毒剂检测技术都有其自身特点。
