Reybrouck G, Werner H P
Zentralbl Bakteriol Orig B. 1975 Jul;160(4-5):392-411.
Four methods for the evaluation of the bactericidal property of disinfectants have been compared. These methods are (1) the qualitative suspension test of the directives for the testing of disinfectants of the German Society for Hygiene and Microbiology, (2) the quantitative suspension test of the dutch committee on phytopharmacy, (3) the carrier or use-dilution method of the A.O.A.C. and (4) the KELSEY and SYKES' capacity test. Aim of this study has been the evaluation of (1) the repeatability and reproducibility of the test results of the four methods, and of (2) the agreement of the degree of efficiency as determined by the different techniques. At least three dilutions of the three disinfectant standards (a phenolic, an aldehyde solution and an iodophore) have been tested against Staphylococcus aureus and Pseudomonas aeruginoas. In this article the authors describe the methods used and the specific difficulties of these techniques. In the suspension technique of the DGHM, the use of only one subculture is stated as not sufficient for exact evaluation of the extinction. In the dutch suspension test, the use of pour plates leads to troubles. The preparative incubation time of the use-dilution method of the A.O.A.C. has been experienced as too long. The structure of the KELSEY and SYKES' test is complicated. Also the results by the two research-teams, are reported and summarized. It has been found that the transition from active to inactive dilution is most marked in the two suspension tests, whereas the distinction between the results of the use-dilution method of the A.O.A.C. is not so clear.
对四种评估消毒剂杀菌性能的方法进行了比较。这些方法分别是：（1）德国卫生与微生物学会消毒剂检测指令中的定性悬液试验；（2）荷兰植物药学委员会的定量悬液试验；（3）美国官方分析化学师协会的载体或使用稀释法；（4）凯尔西和赛克斯容量试验。本研究的目的是评估：（1）这四种方法测试结果的重复性和再现性；（2）不同技术所确定的效率程度的一致性。针对金黄色葡萄球菌和铜绿假单胞菌，对三种消毒剂标准品（一种酚类、一种醛溶液和一种碘伏）的至少三种稀释液进行了测试。在本文中，作者描述了所使用的方法以及这些技术的具体难点。在德国卫生与微生物学会的悬液技术中，仅使用一次传代培养被认为不足以准确评估杀菌效果。在荷兰悬液试验中，倾注平板法会带来问题。美国官方分析化学师协会使用稀释法的预培养时间过长。凯尔西和赛克斯试验的结构复杂。此外，还报告并总结了两个研究团队的结果。结果发现，在两种悬液试验中，从有效稀释到无效稀释的转变最为明显，而美国官方分析化学师协会使用稀释法的结果之间的差异则不那么明显。
