Woolfrey B F, Ramadei W A, Quall C O
Am J Clin Pathol. 1979 Nov;72(5):861-3. doi: 10.1093/ajcp/72.5.861.
The usefulness of the moving intermediate zone concept for improving the performance of the standardized disk agar-diffusion test in measuring susceptibility of the fluorescent group of pseudomonads to gentamicin was studied. For this purpose, a 3-mm moving intermediate zone lying --3 mm and --6 mm below that measured for the quality control microorganism Pseudomonas aeruginosa, ATCC 27853, and a wider moving intermediate zone lying --2 mm and --7 mm below that for the quality control microorganism were investigated. Data from the authors' previous study of the usefulness of using fixed breakpoints and fixed zones for assessing susceptibility of the fluorescent pseudomonads to gentamicin were used for this analysis. The results indicate that both the 3-mm moving intermediate zone and the wider 5-mm moving intermediate zone produced unacceptably high rates of error for testing the susceptibility of the fluorescent group of pseudomonads to gentamicin by the standardized disk agar-diffusion test.
研究了移动中间区概念在改进标准化纸片琼脂扩散试验性能方面的作用，该试验用于测定荧光假单胞菌对庆大霉素的敏感性。为此，研究了一个位于质控微生物铜绿假单胞菌（ATCC 27853）测量值下方3毫米处且移动范围为3毫米的中间区，以及一个位于质控微生物测量值下方2毫米至7毫米处的更宽的移动中间区。作者之前关于使用固定断点和固定区域评估荧光假单胞菌对庆大霉素敏感性的有用性研究数据用于此分析。结果表明，对于通过标准化纸片琼脂扩散试验检测荧光假单胞菌对庆大霉素的敏感性，3毫米移动中间区和更宽的5毫米移动中间区产生的误差率都高得令人无法接受。
