用于检测弯曲杆菌属中与大环内酯类耐药相关点突变的聚合酶链反应-限制性片段长度多态性分析
PCR-restriction fragment length polymorphism analysis for detection of point mutations associated with macrolide resistance in Campylobacter spp.
作者信息
Vacher Sylvie, Ménard Armelle, Bernard Elisabeth, Mégraud Francis
机构信息
Centre National de Référence des Helicobacters et Campylobacters, Laboratoire de Bactériologie, Hopital Pellegrin, Bordeaux, France.
出版信息
Antimicrob Agents Chemother. 2003 Mar;47(3):1125-8. doi: 10.1128/AAC.47.3.1125-1128.2003.
Abstract
A 23S rRNA gene fragment in domain V was sequenced from 30 clinical isolates of Campylobacter spp., including 22 resistant to macrolides. Two point mutations associated with erythromycin resistance were identified at positions 2074 and 2075 on the 23S rRNA gene (homologous to A2142C and A2143G mutations in Helicobacter pylori) in which an adenine residue is also replaced with a cytosine and a guanine residue, respectively. A combined PCR-restriction fragment length polymorphism technique was developed to detect these mutations by using the BsaI and BceAI enzymes.
摘要
对30株弯曲杆菌属临床分离株（包括22株对大环内酯类耐药的菌株）的V结构域中的23S rRNA基因片段进行了测序。在23S rRNA基因的2074和2075位（与幽门螺杆菌中的A2142C和A2143G突变同源）鉴定出两个与红霉素耐药相关的点突变，其中腺嘌呤残基分别被胞嘧啶和鸟嘌呤残基取代。开发了一种联合PCR-限制性片段长度多态性技术，通过使用BsaI和BceAI酶来检测这些突变。
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