Niwa H, Chuma T, Okamoto K, Itoh K
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Int J Antimicrob Agents. 2003 Oct;22(4):374-9. doi: 10.1016/s0924-8579(03)00178-x.
Quinolone and macrolide resistance of Campylobacter jejuni and Campylobacter coli mainly depend on a mutation in gyrA and in 23S rDNA, respectively. In order to detect quinolone and/or macrolide resistant C. jejuni and C. coli strains, a macrolide and quinolone line probe assay (MQ-LiPA) was developed and 42 C. jejuni and C. coli strains were tested to evaluate the efficiency of the assay. Profiles of the mutations in 23S rDNA and in gyrA characterized by MQ-LiPA agreed with resistance to macrolides and quinolones. MQ-LiPA is a rapid and simple method for simultaneous detection of quinolone and macrolide resistance of C. jejuni and C. coli. We could also discriminate between C. jejuni and C. coli using probes for detection of gyrA mutations in MQ-LiPA.
空肠弯曲菌和结肠弯曲菌对喹诺酮类和大环内酯类的耐药性主要分别取决于gyrA和23S rDNA中的突变。为了检测对喹诺酮类和/或大环内酯类耐药的空肠弯曲菌和结肠弯曲菌菌株，开发了一种大环内酯类和喹诺酮类线性探针检测法（MQ-LiPA），并对42株空肠弯曲菌和结肠弯曲菌菌株进行检测以评估该检测法的效率。通过MQ-LiPA表征的23S rDNA和gyrA中的突变谱与对大环内酯类和喹诺酮类的耐药性一致。MQ-LiPA是一种快速简便的方法，可同时检测空肠弯曲菌和结肠弯曲菌对喹诺酮类和大环内酯类的耐药性。我们还可以在MQ-LiPA中使用检测gyrA突变的探针来区分空肠弯曲菌和结肠弯曲菌。
