Kreisler M, Meyer C, Stender E, Daubländer M, Willershausen-Zönnchen B, d'Hoedt B
Department of Oral Surgery, Johannes Gutenberg-University Mainz, Germany.
J Periodontol. 2001 Oct;72(10):1312-7. doi: 10.1902/jop.2001.72.10.1312.
The present study is part of a basic research program investigating the cellular effects of an 810 nm GaAlAs-diode laser on human periodontal tissues. The aim of the investigation was to evaluate the effects of laser treatment of root surface specimens on the attachment of periodontal ligament (PDL) cells in vitro.
Root specimens were prepared from periodontally diseased teeth. PDL cells were obtained from human third molar ligaments. Cells were cultured under simple, standardized, and reproducible experimental conditions. One hundred fifty root specimens were scaled and root planed with curets followed by air-powder abrasive treatment; 75 were then lased and 75 served as controls. The irradiation time was 20 seconds at a power output of 1 W. The root segments were placed into culture dishes, covered with a solution of PDL cells, and incubated for 72 hours. The specimens were then washed with phosphate buffer to remove cells not attached to the surface, and the adherent cells were stained with methylene blue. Cells were counted using a reflected light microscope and the cell density per mm2 was calculated.
The analysis of 150 specimens revealed no significant differences between the groups (P = 0.347, Wilcoxon test). The cell numbers, however, were slightly higher on laser specimens. The mean was 66 cells/mm2 in the laser group and 63.7 cells/mm2 in the control group.
The application of the diode laser at the parameters used did not have a substantially positive effect on the new attachment of PDL cells on the tooth specimens. It remains to be investigated whether the difference detected is really clinically relevant.
本研究是一项基础研究项目的一部分，该项目旨在探究810纳米砷化镓铝二极管激光对人牙周组织的细胞效应。本研究的目的是评估激光处理牙根表面标本对体外牙周膜（PDL）细胞附着的影响。
从患有牙周疾病的牙齿制备牙根标本。从人第三磨牙韧带获取PDL细胞。细胞在简单、标准化且可重复的实验条件下培养。150个牙根标本先用刮匙进行刮治和根面平整，然后进行气粉磨蚀处理；其中75个随后接受激光照射，75个作为对照。输出功率为1瓦时，照射时间为20秒。将牙根段放入培养皿中，覆盖PDL细胞溶液，孵育72小时。然后用磷酸盐缓冲液冲洗标本以去除未附着在表面的细胞，并用亚甲蓝对贴壁细胞进行染色。使用反射光显微镜对细胞进行计数，并计算每平方毫米的细胞密度。
对150个标本的分析显示两组之间无显著差异（P = 0.347，Wilcoxon检验）。然而，激光处理标本上的细胞数量略高。激光组的平均值为66个细胞/平方毫米，对照组为63.7个细胞/平方毫米。
在所使用的参数下应用二极管激光对PDL细胞在牙齿标本上的新附着没有实质性的积极影响。检测到的差异是否真的具有临床相关性仍有待研究。
