Djupesland P G, Qian W, Haight J S
Department of Otorhinolaryngology, Ullevaal University Hospital, Oslo, Norway.
Chest. 2001 Nov;120(5):1645-50. doi: 10.1378/chest.120.5.1645.
The present study introduces a method that has been developed to improve the remote collection and transportation of gas samples from the nose and lungs.
Assessment of agreement between two methods of clinical measurements.
Noninvasive exhaled gas measurement at a respiratory research laboratory.
Ten nonsmoking adult volunteers (median age, 44 years; age range, 33 to 53 years; men, 6; women, 4) were recruited.
Exhaled nitric oxide (ENO) and nasal nitric oxide (NNO) outputs were measured directly (on-line) and remotely (off-line). With the velum closed, lung air was exhaled at fixed flows (ie, 6, 8, and 10 L/min) (ENO) or room-air was aspirated through the nose in series at one fixed flow (ie, 5 to 8 L/min) (NNO). The off-line nitric oxide (NO) measurements were achieved by a gas collection tube system, which consisted of a flow control unit, a tube reservoir with one-way valves at both ends, and an interrupter valve allowing the trapping of gas inside the tube and eliminating the inclusion of "dead space." After clamping, the reservoir may store and transport the gas samples for delayed analysis. The coefficient of variation of three consecutive NO measurements was < 3% for both on-line and off-line ENO and NNO. The correlations between on-line and off-line measurements in both ENO and NNO outputs were high (r = 0.99; R(2) = 0.99), and, unlike previous studies using bag-collection, the ENO outputs for on-line and off-line measurements were in good agreement (Bland-Altman test) at all flows tested.
The tube gas collection system eliminates the dead space and contamination during the gas sampling and permits the cost-effective and reliable off-line collection of both nasal and exhaled gas samples.
本研究介绍了一种已开发出的方法，用于改进从鼻子和肺部远程采集和运输气体样本。
评估两种临床测量方法之间的一致性。
呼吸研究实验室的无创呼出气测量。
招募了10名不吸烟的成年志愿者（年龄中位数为44岁；年龄范围为33至53岁；男性6名；女性4名）。
直接（在线）和远程（离线）测量呼出一氧化氮（ENO）和鼻腔一氧化氮（NNO）输出量。软腭闭合时，以固定流量（即6、8和10升/分钟）呼出肺内气体（ENO），或以一种固定流量（即5至8升/分钟）通过鼻子串联抽吸室内空气（NNO）。离线一氧化氮（NO）测量通过气体收集管系统实现，该系统由流量控制单元、两端带有单向阀的管储器以及允许将气体捕获在管内并消除“死腔”混入的中断阀组成。夹紧后，储器可储存和运输气体样本以供延迟分析。在线和离线ENO及NNO的连续三次NO测量的变异系数均<3%。ENO和NNO输出量的在线和离线测量之间的相关性很高（r = 0.99；R(2) = 0.99），并且与以往使用袋子收集的研究不同，在所有测试流量下，在线和离线测量的ENO输出量都具有良好的一致性（Bland-Altman检验）。
管气体收集系统消除了气体采样过程中的死腔和污染，并允许以经济高效且可靠的方式离线收集鼻腔和呼出气体样本。
