来自假马齿苋的达玛烷三萜皂苷作为多形核细胞中的超氧化物抑制剂。

Dammarane triterpene saponin from Bacopa monniera as the superoxide inhibitor in polymorphonuclear cells.

作者信息

Pawar R, Gopalakrishnan C, Bhutani K K

机构信息

Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Punjab, India.

出版信息

Planta Med. 2001 Nov;67(8):752-4. doi: 10.1055/s-2001-18351.

DOI:10.1055/s-2001-18351

PMID:11731921

Abstract

The hydroalcoholic extract of the whole plant of Bacopa monniera Wettst. (Scrophulariaceae), exhibited an inhibitory effect on superoxide released from polymorphonuclear (PMN) cells in the nitroblue tetrazolium (NBT) assay. The major saponin bacoside A(3) was found to be responsible for this effect in the herb. This compound showed 85, 91.66, 91.66, and 83 % inhibitions of NBT reduction at the concentrations of 200, 100, 50, and 25 microg/ml, respectively, with an IC(50) value of 10.22 microg/ml. These inhibitory effects were compared with those of the standard positive controls, quercetin and ascorbic acid with IC(50) of 111 and 14.16 microg/ml, respectively. Another major saponin bacopasaponin C was found to be much less potent as compared to bacoside A(3) whereas the remaining two mixtures of saponins were found to be inactive.

摘要

假马齿苋（玄参科）全草的水醇提取物在氮蓝四唑（NBT）试验中对多形核（PMN）细胞释放的超氧化物具有抑制作用。发现该草药中的主要皂苷Bacoside A(3)是造成这种作用的原因。该化合物在浓度分别为200、100、50和25μg/ml时对NBT还原的抑制率分别为85%、91.66%、91.66%和83%，IC(50)值为10.22μg/ml。将这些抑制作用与标准阳性对照槲皮素和抗坏血酸的抑制作用进行比较，槲皮素和抗坏血酸的IC(50)分别为111和14.16μg/ml。发现另一种主要皂苷Bacosapaponin C的效力远低于Bacoside A(3)，而其余两种皂苷混合物则无活性。