Wang L, Cull G, Cioffi G A
Discoveries in Sight, Devers Eye Institute 1225 NE Second Ave, Portland, OR 97232, USA.
Arch Ophthalmol. 2001 Dec;119(12):1810-4. doi: 10.1001/archopht.119.12.1810.
To estimate the measuring depth of the blood flow and to establish the vascular contributions to these measurements with scanning laser Doppler flowmetry (SLDF) of the primate anterior optic nerve.
Optic nerve blood flow in each eye of 8 monkeys was measured using SLDF before and following surgical occlusion of the central retinal artery (n = 4) or posterior ciliary arteries (n = 4). The regional blood flow in both eyes was determined using a nonradioactive microsphere method.
The blood flow in the nerve fiber layer (NFL), including the prelaminar region, was measured with microspheres after central retinal artery occlusion; it was significantly reduced (-83%) with no significant change in the combined laminar and retrolaminar regions. The blood flow measured with SLDF had a 51% reduction. After posterior ciliary artery occlusion, the blood flow in the NFL was measured with microspheres and was not significantly affected (+2%); neither was that measured with SLDF (-12%). However, there was a 51% reduction in the laminar and retrolaminar regions when microspheres were used. The mean +/- SD tissue thickness of the NFL was 359 +/- 16 microm and 353 +/- 54 microm in each group.
Scanning laser Doppler flowmetry measures blood flow principally in the NFL of the anterior optic nerve, which is primarily supplied by the central retinal artery. Blood flow in the laminar and retrolaminar regions makes a small contribution to the SLDF measurement, with an NFL thickness between 300 and 400 microm.
Scanning laser Doppler flowmetry is used for the noninvasive evaluation of ocular microcirculation in diseases such as glaucoma. Because of the dual blood flow supply in the optic nerve and the limited penetration power of the laser, the instrument primarily measures the microcirculation on the surface of the optic nerve, which is largely supplied by the central retinal artery rather than the ciliary arteries.
利用扫描激光多普勒血流仪（SLDF）测量灵长类动物视神经前部的血流深度，并确定这些测量中的血管贡献。
在8只猴子的每只眼睛中，于视网膜中央动脉（n = 4）或睫状后动脉（n = 4）手术闭塞前后，使用SLDF测量视神经血流。使用非放射性微球法测定双眼的局部血流。
视网膜中央动脉闭塞后，用微球测量包括神经纤维层前层区域在内的神经纤维层（NFL）血流；血流显著减少（-83%），而层状和层后区域血流无显著变化。用SLDF测量的血流减少了51%。睫状后动脉闭塞后，用微球测量NFL血流且未受显著影响（+2%）；用SLDF测量的血流也未受显著影响（-12%）。然而，使用微球时层状和层后区域血流减少了51%。每组中NFL的平均±标准差组织厚度分别为359±16微米和353±54微米。
扫描激光多普勒血流仪主要测量视神经前部NFL的血流，该区域主要由视网膜中央动脉供血。层状和层后区域的血流对SLDF测量贡献较小，NFL厚度在300至400微米之间。
扫描激光多普勒血流仪用于青光眼等疾病中眼微循环的无创评估。由于视神经存在双重血流供应且激光穿透能力有限，该仪器主要测量视神经表面的微循环，其主要由视网膜中央动脉而非睫状动脉供血。
