Sullivan N M, Sutter V L, Finegold S M
J Clin Microbiol. 1975 Jan;1(1):37-43. doi: 10.1128/jcm.1.1.37-43.1975.
During the course of preliminary clinical trials of an improved membrane filter blood culture system, filter plugging produced by a gelling of the lysing solution was observed when the patients had high leukocyte counts. A solution of streptokinase-streptodornase (Varidase, Lederle Laboratories) dissolved or prevented the gel and permitted rapid filtration without plugging. With streptokinase-streptodornase incorporated in the filtration procedure, a comparison of several culture systems was carried out on 176 blood cultures. Brucella broth with and without sodium polyanethol sulfonate, a prereduced osmotically stabilized broth, pour plates, and an improved aerobic membrane filter system were compared. The membrane filter system yielded 29 of the total of 37 positive cultures, far surpassing all other systems. Eight of these cultures were detected first by the filter technique, and 13 were positive only in this system. Nineteen of the 37 positive cultures were from patients on antimicrobial agents. Fourteen of these were detected by the filter, twice the number detected by any of the other systems.
在一种改良的膜滤器血培养系统的初步临床试验过程中，当患者白细胞计数较高时，观察到裂解溶液凝胶化导致滤器堵塞。链激酶 - 链道酶溶液（Varidase，莱德利实验室）溶解或防止了凝胶形成，并允许快速过滤而不堵塞。将链激酶 - 链道酶纳入过滤程序后，对176份血培养物进行了几种培养系统的比较。比较了含和不含聚茴香脑磺酸钠的布鲁氏菌肉汤、一种预还原的渗透稳定肉汤、倾注平板法以及一种改良的需氧膜滤器系统。膜滤器系统在总共37份阳性培养物中检出29份，远远超过所有其他系统。其中8份培养物首先通过滤器技术检测到，13份仅在该系统中呈阳性。37份阳性培养物中有19份来自使用抗菌剂的患者。其中14份通过滤器检测到，是其他任何系统检测到数量的两倍。
