Appleman M D, Swinney R S, Heseltine P N
J Clin Microbiol. 1982 Feb;15(2):278-81. doi: 10.1128/jcm.15.2.278-281.1982.
A new system called an Antibiotic Removal Device (ARD), used for the removal of residual antibiotics in blood specimens, was studied in 300 patients diagnosed as clinically septic despite antimicrobial therapy. Blood specimens from these patients were processed with and without the ARD into aerobic and anaerobic media. There were 53 patients who had one or more positive blood culture bottles, for a total of 109 positive blood culture bottles. Of these 109 bottles, 33.9% were positive with ARD-processed blood in aerobic media, 24.8% with ARD-processed blood in anaerobic media, 22.9% with conventionally processed blood in aerobic media, and 18.3% with conventionally processed specimens in anaerobic media. After 6 h of incubation, 14 bottles with blood processed by the ARD and 9 bottles processed conventionally were positive; after 24 h, 48 bottles with blood processed by the ARD and 31 bottles processed conventionally were positive. The same organisms were isolated from both ARD- and conventionally processed blood, with four exceptions. The ARD system, when compared with a conventional system of blood specimen processing, significantly increased the detection of bacteremia and decreased the time required for its detection in patients clinically septic despite ongoing antimicrobial therapy.
一种名为抗生素去除装置（ARD）的新系统，用于去除血液标本中的残留抗生素，该系统在300名尽管接受了抗菌治疗但临床诊断为败血症的患者中进行了研究。对这些患者的血液标本分别使用ARD和不使用ARD进行处理，并接种到需氧和厌氧培养基中。有53名患者的一个或多个血培养瓶呈阳性，总共109个血培养瓶阳性。在这109个培养瓶中，需氧培养基中经ARD处理的血液培养瓶阳性率为33.9%，厌氧培养基中经ARD处理的血液培养瓶阳性率为24.8%，需氧培养基中传统处理的血液培养瓶阳性率为22.9%，厌氧培养基中传统处理的标本阳性率为18.3%。培养6小时后，经ARD处理的血液有14个培养瓶阳性，传统处理的有9个培养瓶阳性；培养24小时后，经ARD处理的血液有48个培养瓶阳性，传统处理的有31个培养瓶阳性。除了4个例外情况，从经ARD处理和传统处理的血液中分离出的是相同的微生物。与传统的血液标本处理系统相比，ARD系统显著提高了菌血症的检测率，并且缩短了在尽管正在进行抗菌治疗但临床败血症患者中检测菌血症所需的时间。
