Meurman O H, Viljanen M K, Granfors K
J Clin Microbiol. 1977 Mar;5(3):257-62. doi: 10.1128/jcm.5.3.257-262.1977.
The solid-phase radioimmunoassay (RIA) method developed in our laboratory for demonstrating rubella virus-specific immunoglobulin G (IgG) antibodies (Kalimo et al., 1976) was further developed for demonstrating IgM antibodies. A total of 188 serum specimens were tested. The statistical probability of obtaining a false-positive IgM result, based on determinations of 100 rubella-negative sera, was below 0.001. Nonspecific inhibitors and IgM antibodies against other viruses tested did not interfere in the assay. In 2 out of 20 (10%) serum specimens with rheumatoid factor, a false-positive IgM result was obtained. The new RIA method was compared with sucrose density gradient centrifugation, followed by hemagglutination inhibition testing of the separated immunoglobulins with respect to demonstrating IgM antibodies. In patients with acute rubella infection, IgM antibodies were demonstrated by RIA in 9 out of 20 acute-phase sera and in all 20 early-convalescent-phase sera, compared with 7 out of 20 acute-phase sera and 19 out of 20 early-convalescent-phase sera by sucrose density gradient centrifugation. The results obtained indicate that the RIA method is reliable and sensitive and suitable for routine diagnostic use.
我们实验室开发的用于检测风疹病毒特异性免疫球蛋白G（IgG）抗体的固相放射免疫测定（RIA）方法（卡里莫等人，1976年）进一步发展用于检测IgM抗体。共检测了188份血清标本。根据对100份风疹阴性血清的检测，获得假阳性IgM结果的统计概率低于0.001。非特异性抑制剂和针对其他检测病毒的IgM抗体在该检测中不产生干扰。在20份含有类风湿因子的血清标本中有2份（10%）获得了假阳性IgM结果。将新的RIA方法与蔗糖密度梯度离心法进行比较，随后对分离的免疫球蛋白进行血凝抑制试验以检测IgM抗体。在急性风疹感染患者中，通过RIA在20份急性期血清中的9份以及所有20份早期恢复期血清中检测到IgM抗体，相比之下，通过蔗糖密度梯度离心法在20份急性期血清中的7份以及20份早期恢复期血清中的19份检测到IgM抗体。所获得的结果表明，RIA方法可靠、灵敏且适用于常规诊断用途。
