Wang Q, Lin J, Sun H
Department of Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2000 Mar;23(3):147-50.
To investigate the changes of T helper cell(TH) subsets and relationship to the relative cytokines, serum total IgE, eosinophil count and ventilatory function in asthmatic patients.
20 asthmatic patients and 10 healthy subjects were selected. The counts of the TH subsets, the level of IL-4 and IFN-gamma in the supernatant of peripheral blood mononuclear cell(PBMC), serum total IgE concentration and eosinophil count in peripheral blood were determined in acute attack and stable patients with asthma and healthy subjects.
The TH2/TH1 ratio in the acute attack and the stable patients with asthma were increased significantly, compared with that in normal group (P < 0.01). The relation analysis showed that the value of TH1 had significantly positive relation with that of IFN-gamma, the value of TH2 had significantly positive relation with that of IL-4. The total serum IgE had significantly positive relation with the level of IL-4. FEV1 had significantly positive relation with that of TH1, negative relation with that of TH2, and TH2/TH1 significantly. The total serum IgE and the number of eosinophils had significantly positive relation with that of TH2.
The balance of TH in asthmatic patients deviates significantly. The deviation of balance of TH has relation with severity of asthma.
探讨哮喘患者T辅助细胞（TH）亚群的变化及其与相关细胞因子、血清总IgE、嗜酸性粒细胞计数和通气功能的关系。
选取20例哮喘患者和10名健康对照者。测定哮喘急性发作期和缓解期患者及健康对照者外周血单个核细胞（PBMC）上清液中TH亚群计数、IL-4和IFN-γ水平、血清总IgE浓度及外周血嗜酸性粒细胞计数。
哮喘急性发作期和缓解期患者的TH2/TH1比值较正常对照组显著升高（P < 0.01）。相关性分析显示，TH1值与IFN-γ值呈显著正相关，TH2值与IL-4值呈显著正相关。血清总IgE与IL-4水平呈显著正相关。FEV1与TH1值呈显著正相关，与TH2值呈显著负相关，与TH2/TH1比值呈显著负相关。血清总IgE和嗜酸性粒细胞计数与TH2值呈显著正相关。
哮喘患者TH平衡明显失调。TH平衡失调与哮喘严重程度有关。
