Kalaydjiev Svetoslav, Dimitrova Dimitrina, Nenova Marina, Peneva Siika, Dikov Ivan, Nakov Lyudmi
Department of Biology, Medical University of Sofia, Bulgaria.
Fertil Steril. 2002 Jan;77(1):76-82. doi: 10.1016/s0015-0282(01)02926-0.
To assess the level of serum sperm antibodies after mumps orchitis.
Controlled descriptive study.
Academic research environment.
PATIENT(S): Seventy-four mumps orchitis patients.
INTERVENTION(S): Sampling of serum at different intervals after the onset of orchitis symptoms: 1 to 7 days, 31 to 60 days, and 61 to 431 days.
MAIN OUTCOME MEASURE(S): Level of serum sperm antibodies, using Kibrick's gelatin agglutination test, Friberg's tray agglutination test, Isojima's sperm immobilization test, and ELISA.
RESULT(S): Clinically relevant sperm antibody values were detected by the Friberg method among patients tested from 1 to 7 days (10.5%) and 61 to 431 days (10.5%) after the onset of disease. The Isojima test revealed a statistically insignificant higher incidence among patients at 61 to 431 days (31.6%) as compared with those sampled at 1 to 7 days (10.5%). None of the orchitis sera tested positive by the Kibrick and ELISA techniques. The established incidences did not differ significantly from the results for negative controls (blood donors) and were lower than the values acquired from positive controls (males with unexplained infertility).
CONCLUSION(S): Mumps orchitis does not cause enhanced humoral immunity to spermatozoa.
评估流行性腮腺炎睾丸炎患者血清精子抗体水平。
对照描述性研究。
学术研究环境。
74例流行性腮腺炎睾丸炎患者。
在睾丸炎症状出现后的不同时间点采集血清样本：发病后1至7天、31至60天以及61至431天。
采用基布里克明胶凝集试验、弗里伯格平板凝集试验、矶岛精子制动试验和酶联免疫吸附测定法检测血清精子抗体水平。
采用弗里伯格法检测发现，发病后1至7天检测的患者（10.5%）以及61至431天检测的患者（10.5%）中出现了具有临床意义的精子抗体值。矶岛试验显示，发病后61至431天检测的患者（31.6%）精子抗体发生率高于1至7天检测的患者（10.5%），但差异无统计学意义。基布里克法和酶联免疫吸附测定法检测的睾丸炎血清样本均未呈阳性。既定的发生率与阴性对照（献血者）的结果无显著差异，且低于阳性对照（不明原因不育男性）的值。
流行性腮腺炎睾丸炎不会导致对精子的体液免疫增强。
