葡萄糖、乙酰乙酸与胰岛素在泌乳大鼠乳腺切片中的相互作用
Interactions of glucose, acetoacetate and insulin in mammary-gland slices of lactating rats.
作者信息
Williamson D H, McKeown S R, Ilic V
出版信息
Biochem J. 1975 Aug;150(2):145-52. doi: 10.1042/bj1500145.
Abstract
- Utilization of 5mM-glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhibition was accompanied by increases in the intracellular concentrations of citrate and glucose 6-phosphate. 2. In the presence of acetoacetates the accumulation of pyruvate in the medium approximately doubled. 3. Insulin completely reversed the inhibitory effect of acetoacetates on glucose utilization, without altering the amount of acetoacetate removed or pyruvate formed. 4. Similar results were obtained with mammary-gland slices from diabetic rats, except that insulin did not completely reverse the effects of acetoacetates. 5. Acetoacetate inhibited the formation of 14CO2 from [1-14C]pyruvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C]acetoacetate that was converted into lipid and decreased that oxidized to CO2.7. The physiological significance of these findings is discussed.
摘要
- 向孵育培养基中添加乙酰乙酸（2mM）后，泌乳期乳腺切片对5mM葡萄糖的利用率降低了33%。这种抑制作用伴随着细胞内柠檬酸和6-磷酸葡萄糖浓度的增加。2. 在存在乙酰乙酸的情况下，培养基中丙酮酸的积累量大约增加了一倍。3. 胰岛素完全逆转了乙酰乙酸对葡萄糖利用的抑制作用，而不改变乙酰乙酸的去除量或丙酮酸的生成量。4. 用糖尿病大鼠的乳腺切片也得到了类似的结果，只是胰岛素并没有完全逆转乙酰乙酸的作用。5. 乙酰乙酸抑制了[1-14C]丙酮酸生成14CO2；胰岛素无法克服这种作用。6. 胰岛素增加了[3-14C]乙酰乙酸转化为脂质的比例，并降低了其氧化为CO2的比例。7. 讨论了这些发现的生理学意义。
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