Fautz E, Rosenfelder G, Grotjahn L
J Bacteriol. 1979 Dec;140(3):852-8. doi: 10.1128/jb.140.3.852-858.1979.
The fatty acids present in the total hydrolysates of several gliding bacteria (Myxococcus fulvus, Stigmatella aurantiaca, Cytophaga johnsonae, Cytophaga sp. strain samoa and Flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. In addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. The majority was odd-numbered and iso-branched. Small amounts of even-numbered and unbranched fatty acids were also present. Whereas 2-hydroxy-15-methyl hexadecanoic acid was characteristic for myxobacteria, 2-hydroxy-13-methyl-tetradecanoic acid, 3-hydroxy-13-methyl-tetradecanoic acid, and 3-hydroxy-15-methyl-hexadecanoic acid were dominant in the Cytophaga-Flexibacter group.
通过气相色谱-质谱联用技术分析了几种滑行细菌(黄色粘球菌、橙黄标桩菌、约翰逊噬纤维菌、萨摩亚噬纤维菌菌株和优美屈挠杆菌)总水解产物中的脂肪酸。除了13-甲基十四烷酸、15-甲基十六烷酸、十六烷酸和十六碳烯酸外,2-羟基脂肪酸和3-羟基脂肪酸占总脂肪酸的比例高达50%。大多数为奇数碳且具有异分支结构。也存在少量偶数碳且无分支的脂肪酸。2-羟基-15-甲基十六烷酸是粘细菌的特征性脂肪酸,而2-羟基-13-甲基十四烷酸、3-羟基-13-甲基十四烷酸和3-羟基-15-甲基十六烷酸在噬纤维菌-屈挠杆菌组中占主导地位。