Sun Wen-Bing, Han Ben-Li, Peng Zhi-Ming, Li Kun, Ji Qiang, Chen Juan, Wang Huai-Zhi, Ma Rui-Liang
World J Gastroenterol. 1998 Feb;4(1):77-79. doi: 10.3748/wjg.v4.i1.77.
To investigate the age-related alterations of cytoskeleton system in liver Kupffer cell and their relation to the changed phagocytic function.METHODS: The phagocytic function of Kupffer cells from rats of various ages (6mo, 12mo,18mo and 24mo) were quantitatively evaluated by phagocytosis of polystyrene beads. The actin distribution and measurement of Kupffer cell were determined by a phalloidin-TRITC method; and the myosin and vimentin distribution and measurement with indirect immunochemical staining.RESULTS: Aging resulted in significant alterations of actin, myosin and vimentin distributions and reductions in Kupffer cell; the 3 cytoskeleton components of 24-mo-old Kupffer cell were significantly decreased to 68.0%, 84.9% and 75.5%, respectively of these of 6-mo-old Kupffer cell(P < 0.01,0.01 and 0.01). And these decreases had significant positive relations with the damaged phagocytosis of the aged Kupffer cell.gammavalues were 0.96(P < 0.05), 0.99(P < 0.01) and 0.95 (P < 0.05) respectively.CONCLUSION: The cytos-keleton system of the aged Kupffer cell presents an evident state of senescence, which may be an important mechanism of decreased phagocytosis of the aged Kupffer cell.
研究肝库普弗细胞细胞骨架系统随年龄的变化及其与吞噬功能改变的关系。方法：通过聚苯乙烯珠吞噬试验对不同年龄（6个月、12个月、18个月和24个月）大鼠的库普弗细胞吞噬功能进行定量评估。采用鬼笔环肽-TRITC法测定库普弗细胞肌动蛋白分布及含量；采用间接免疫化学染色法测定肌球蛋白和波形蛋白分布及含量。结果：衰老导致库普弗细胞肌动蛋白、肌球蛋白和波形蛋白分布显著改变且含量降低；24月龄库普弗细胞的这3种细胞骨架成分分别显著降至6月龄库普弗细胞的68.0%、84.9%和75.5%（P<0.01、0.01和0.01）。这些降低与衰老库普弗细胞吞噬功能受损呈显著正相关，γ值分别为0.96（P<0.05）、0.99（P<0.01）和0.95（P<0.05）。结论：衰老库普弗细胞的细胞骨架系统呈现明显的衰老状态，这可能是衰老库普弗细胞吞噬功能下降的重要机制。
