用于灵敏快速检测肠道病毒的工程化BGMK细胞。
Engineered BGMK cells for sensitive and rapid detection of enteroviruses.
作者信息
Huang Yung T, Yam Paul, Yan Huimin, Sun Yan
机构信息
Department of Pathology, University Hospital of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
J Clin Microbiol. 2002 Feb;40(2):366-71. doi: 10.1128/JCM.40.2.366-371.2002.
Abstract
Decay-accelerating factor (DAF) has been reported to be a cellular receptor for several enteroviruses. Buffalo green monkey kidney (BGMK) cells expressing human DAF (BGMK-hDAF cells) showed increased susceptibility and sensitivity to several types of enteroviruses compared to wild-type BGMK cells. When 17 frozen positive clinical samples were tested, BGMK cells detected 8 and BGMK-hDAF cells detected 16. Since the CaCo-2 cell line has been documented to support the replication of most enteroviruses, CaCo-2 cells were mixed with BGMK-hDAF cells in order to increase the number of viruses detected. Thirty-four frozen clinical samples that previously had tested positive for enteroviruses were tested, and the following numbers were detected: 33 of 34 by CaCo-2/BGMK-hDAF cells, 29 of 34 by CaCo-2/BGMK cells, 28 of 34 by H292/RD (E-mix A) and A-549/BGMK (E-mix B) cells, and 26 of 34 by MRC-5 and pRhMK cells.
摘要
衰变加速因子（DAF）据报道是几种肠道病毒的细胞受体。与野生型水牛绿猴肾（BGMK）细胞相比，表达人DAF的BGMK细胞（BGMK-hDAF细胞）对几种类型的肠道病毒表现出更高的易感性和敏感性。当检测17份冷冻的阳性临床样本时，BGMK细胞检测出8份，BGMK-hDAF细胞检测出16份。由于已证明CaCo-2细胞系能支持大多数肠道病毒的复制，因此将CaCo-2细胞与BGMK-hDAF细胞混合以增加检测到的病毒数量。对之前检测出肠道病毒呈阳性的34份冷冻临床样本进行了检测，检测到的数量如下：CaCo-2/BGMK-hDAF细胞检测出34份中的33份，CaCo-2/BGMK细胞检测出34份中的29份，H292/RD（E-mix A）和A-549/BGMK（E-mix B）细胞检测出34份中的28份，MRC-5和pRhMK细胞检测出34份中的26份。
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