嗜水气单胞菌与急性腹泻病：肠毒素检测及菌株生物分型
Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains.
作者信息
Ljungh A, Popoff M, Wadstrom T
出版信息
J Clin Microbiol. 1977 Aug;6(2):96-100. doi: 10.1128/jcm.6.2.96-100.1977.
Abstract
Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be inactivated upon heating at 56 degrees C or by specific antihemolysin. Biotyping of each isolate was performed with a conventional test system and with API 50E and APIZYM kit systems (Analytab, Inc.). No single test of the more than 70 biochemical reactions investigated was found to correlate with enterotoxigenicity in the strains of Aeromonas hydrophila examined. All strains were found to belong to ideal phenotypes of A. hydrophila, but each strain possessed its own biochemical profile.
摘要
通过兔肠袢、兔皮肤及肾上腺Y1细胞对培养滤液进行检测，发现从人类粪便培养物中分离出的11株气单胞菌属菌株可产生肠毒素活性。溶血素和一种细胞毒性蛋白在所有三种检测系统中均有干扰作用，但在56℃加热或使用特异性抗溶血素后可失活。使用传统检测系统以及API 50E和APIZYM试剂盒系统（Analytab公司）对每株分离菌进行生物分型。在所检测的70多种生化反应中，未发现任何单一检测与嗜水气单胞菌菌株的产肠毒素性相关。所有菌株均属于嗜水气单胞菌的理想表型，但每个菌株都有其自身的生化特征。
相似文献
1
Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains.嗜水气单胞菌与急性腹泻病：肠毒素检测及菌株生物分型
J Clin Microbiol. 1977 Aug;6(2):96-100. doi: 10.1128/jcm.6.2.96-100.1977.
2
Enterotoxigenicity, hemagglutination and cell-surface hydrophobicity in Aeromonas hydrophila, A. sobria and A. salmonicida.嗜水气单胞菌、温和气单胞菌和杀鲑气单胞菌中的产肠毒素性、血凝作用及细胞表面疏水性
Vet Microbiol. 1983 Feb;8(1):17-34. doi: 10.1016/0378-1135(83)90016-0.
3
Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species.气单胞菌属的分类分组及分离来源与肠毒素产生的关系。
J Clin Microbiol. 1984 Feb;19(2):175-80. doi: 10.1128/jcm.19.2.175-180.1984.
4
Aeromonas spp. and their association with human diarrheal disease.气单胞菌属及其与人类腹泻病的关联。
J Clin Microbiol. 1991 May;29(5):853-6. doi: 10.1128/jcm.29.5.853-856.1991.
5
Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease.嗜水气单胞菌产生的细胞毒性肠毒素：产毒菌株与腹泻病的关系。
Infect Immun. 1979 Mar;23(3):829-37. doi: 10.1128/iai.23.3.829-837.1979.
6
Haemagglutinating activity, serum sensitivity and enterotoxigenicity of Aeromonas spp.气单胞菌属的血凝活性、血清敏感性及产肠毒素性
J Med Microbiol. 1993 Jan;38(1):49-53. doi: 10.1099/00222615-38-1-49.
7
Biochemical characteristics and enterotoxicity of Aeromonas species isolated from man and environment.从人和环境中分离出的气单胞菌属的生化特性及肠毒性
J Diarrhoeal Dis Res. 1992 Dec;10(4):231-4.
8
Evidence for production of an enterotoxin and cholera toxin cross-reactive factor by Aeromonas hydrophila.嗜水气单胞菌产生肠毒素和霍乱毒素交叉反应因子的证据。
J Clin Microbiol. 1986 Oct;24(4):661-4. doi: 10.1128/jcm.24.4.661-664.1986.
9
Clinical and biochemical significance of toxin production by Aeromonas hydrophila.嗜水气单胞菌毒素产生的临床和生化意义。
J Clin Microbiol. 1987 May;25(5):916-21. doi: 10.1128/jcm.25.5.916-921.1987.
10
Multiplex PCR detection of enterotoxin genes in Aeromonas spp. from suspect food samples in northern Taiwan.台湾北部疑似食品样本中气单胞菌属肠毒素基因的多重聚合酶链反应检测
J Food Prot. 2008 Oct;71(10):2094-9. doi: 10.4315/0362-028x-71.10.2094.
引用本文的文献
1
An estimation of global Aeromonas infection prevalence in children with diarrhoea: a systematic review and meta-analysis.全球腹泻儿童中气单胞菌感染流行率的估计：系统评价和荟萃分析。
BMC Pediatr. 2023 May 22;23(1):254. doi: 10.1186/s12887-023-04081-3.
2
A review on pathogenicity of and their mitigation through medicinal herbs in aquaculture.水产养殖中病原体的致病性及其通过草药减轻的研究综述。
Heliyon. 2023 Feb 28;9(3):e14088. doi: 10.1016/j.heliyon.2023.e14088. eCollection 2023 Mar.
3
Low Prevalence of Infectious Diarrhea Samples of Pediatric Patients in Arak, Iran.伊朗阿拉克市儿科患者感染性腹泻样本的低患病率
Rep Biochem Mol Biol. 2016 Oct;5(1):15-19.
4
Identification of opportunistic pathogenic bacteria in drinking water samples of different rural health centers and their clinical impacts on humans.不同农村卫生中心饮用水样本中的机会性病原体鉴定及其对人类的临床影响。
Biomed Res Int. 2013;2013:348250. doi: 10.1155/2013/348250. Epub 2013 Jun 3.
5
Isolation of carbohydrate-reactive outer membrane proteins of Aeromonas hydrophila.嗜水气单胞菌碳水化合物反应性外膜蛋白的分离
Infect Immun. 1993 Feb;61(2):371-7. doi: 10.1128/iai.61.2.371-377.1993.
6
Isolation, enumeration, and characterization of Aeromonas from polluted waters encountered in diving operations.从潜水作业中遇到的受污染水体中分离、计数和气单胞菌的鉴定
Appl Environ Microbiol. 1980 May;39(5):1010-8. doi: 10.1128/aem.39.5.1010-1018.1980.
7
Medium for the accumulation of extracellular hemolysin and protease by Aeromonas hydrophila.嗜水气单胞菌积累细胞外溶血素和蛋白酶的培养基。
Infect Immun. 1981 Sep;33(3):728-33. doi: 10.1128/iai.33.3.728-733.1981.
8
Enterotoxigenic bacteria in food and water from an Ethiopian community.来自埃塞俄比亚一个社区的食物和水中的产肠毒素细菌。
Appl Environ Microbiol. 1981 Apr;41(4):1010-9. doi: 10.1128/aem.41.4.1010-1019.1981.
9
Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes and yeast cells.基于与红细胞和酵母细胞凝集模式的嗜水气单胞菌分型方案。
J Clin Microbiol. 1983 Mar;17(3):422-7. doi: 10.1128/jcm.17.3.422-427.1983.
10
Enzymatic characterization of Aeromonas hydrophila complex by the API ZYM system.应用API ZYM系统对嗜水气单胞菌复合体进行酶学特性分析。
J Clin Microbiol. 1982 Oct;16(4):692-6. doi: 10.1128/jcm.16.4.692-696.1982.
本文引用的文献
1
The genus aeromonas in human bacteriology; report of 30 cases and review of the literature.人类细菌学中的气单胞菌属；30例报告及文献综述
N Engl J Med. 1968 Feb 1;278(5):245-9. doi: 10.1056/NEJM196802012780504.
2
An enterotoxin of Pseudomonas aeruginosa.铜绿假单胞菌的一种肠毒素。
J Infect Dis. 1971 Jan;123(1):97-8. doi: 10.1093/infdis/123.1.97.
3
Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing.通过等电聚焦从嗜水气单胞菌中分离出两种溶血素。
Infect Immun. 1971 Oct;4(4):503-5. doi: 10.1128/iai.4.4.503-505.1971.
4
The detection of Aeromonas hydrophila in stool specimens.粪便标本中嗜水气单胞菌的检测
Health Lab Sci. 1970 Jul;7(3):124-7.
5
Tissue-culture assay of antibodies to heat-labile Escherichia coli enterotoxins.对不耐热大肠杆菌肠毒素抗体的组织培养测定
N Engl J Med. 1974 Jul 18;291(3):117-21. doi: 10.1056/NEJM197407182910302.
6
Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin.霍乱弧菌与大肠杆菌不耐热肠毒素在兔回肠和皮肤中和试验中的交叉反应性
Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757-62. doi: 10.1111/j.1699-0463.1973.tb02272.x.
7
Three characteristics associated with enterotoxigenic Escherichia coli isolated from man.从人类分离出的产肠毒素大肠杆菌的三个相关特征。
Infect Immun. 1973 Sep;8(3):322-8. doi: 10.1128/iai.8.3.322-328.1973.
8
Stimulation of jejunal secretion by a crude Escherichia coli enterotixin.一种粗制大肠杆菌肠毒素对空肠分泌的刺激作用。
Gastroenterology. 1972 Sep;63(6):439-48.
9
Plesiomonas shigelloides. Survey and possible association with diarrhoea in Zaïre.
Ann Soc Belg Med Trop. 1974;54(6):503-13.
10
The characterization and significance of Plesiomonas shigelloides and Aeromonas hydrophila isolated from an epidemic of diarrhoea.从一次腹泻流行中分离出的类志贺邻单胞菌和嗜水气单胞菌的特性及意义。
Indian J Med Res. 1974 Jul;62(7):1051-60.
Zotero 插件