Péter Olivier, Bretz Anne-Gabrielle, Postic Danièle, Dayer Eric
Infectious Diseases and Immunology, Institut Central des Hôpitaux Valaisans, Sion, Switzerland.
Clin Microbiol Infect. 1997 Aug;3(4):423-431. doi: 10.1111/j.1469-0691.1997.tb00278.x.
To evaluate by species-specific immunoblots the association of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii with neuroborreliosis in Switzerland. METHODS: Borrelia strains isolated from the cerebrospinal fluid (CSF) of three children with neuroborreliosis were typed by phenotypic and genotypic analysis. The serologic reactions (IgG) of these three patients as well as those of 28 patients, including one of these three children, with confirmed neuroborreliosis were characterized and scored by immunoblots on the three individual Borrelia species antigens. Twenty patients with typical erythema migrans served as a control group. RESULTS: Phenotypic and genotypic analysis confirmed that all three CSF isolates were B. garinii. In the 28 patients with neuroborreliosis, the comparatively strongest reactions were as follows: 18 to B. garinii, three to B. burgdorferi sensu stricto and two to B. afzelii; five were inconclusive. In the control group (erythema migrans), the comparatively strongest reactions were as follows: six B. garinii, one to B. burgdorferi sensu stricto and five to B. afzelii; eight were indeterminate. CONCLUSIONS: Typing of these three CSF isolates and characterization by immunoblots of the antibody reactions of patients with neuroborreliosis give additional evidence of the association of B. garinii and neuroborreliosis. Our serologic results suggest that B. burgdorferi sensu stricto and B. afzelii are also responsible for some neuroborreliosis cases in Switzerland. Our immunoblots and the scoring system proved particularly useful for the serologic typing of patients with late Lyme borreliosis.
通过种特异性免疫印迹法评估狭义伯氏疏螺旋体、伽氏疏螺旋体和阿氏疏螺旋体与瑞士神经莱姆病的关联。方法：对从3例神经莱姆病患儿脑脊液（CSF）中分离出的伯氏疏螺旋体菌株进行表型和基因型分析。通过对三种伯氏疏螺旋体抗原进行免疫印迹，对这3例患者以及另外28例确诊为神经莱姆病的患者（包括这3例患儿中的1例）的血清学反应（IgG）进行特征分析和评分。20例典型游走性红斑患者作为对照组。结果：表型和基因型分析证实，所有3株脑脊液分离株均为伽氏疏螺旋体。在28例神经莱姆病患者中，相对最强反应如下：18例对伽氏疏螺旋体，3例对狭义伯氏疏螺旋体，2例对阿氏疏螺旋体；5例结果不明确。在对照组（游走性红斑）中，相对最强反应如下：6例对伽氏疏螺旋体，1例对狭义伯氏疏螺旋体，5例对阿氏疏螺旋体；8例结果不确定。结论：对这3株脑脊液分离株进行分型，并通过免疫印迹对神经莱姆病患者的抗体反应进行特征分析，进一步证明了伽氏疏螺旋体与神经莱姆病的关联。我们的血清学结果表明，狭义伯氏疏螺旋体和阿氏疏螺旋体在瑞士的一些神经莱姆病病例中也起作用。我们的免疫印迹和评分系统被证明对晚期莱姆病伯氏疏螺旋体病患者的血清学分型特别有用。
