Sivamani Elumalai, Brey Christopher W, Talbert Luther E, Young Mark A, Dyer William E, Kaniewski Wojciech K, Qu Rongda
Department of Plant Sciences, Montana State University, Bozeman 59717-3140, USA.
Transgenic Res. 2002 Feb;11(1):31-41. doi: 10.1023/a:1013944011049.
Wheat (Triticum aestivum) plants were stably transformed with the coat protein (CP) gene of wheat streak mosaic virus (WSMV) by the biolistic method. Eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to WSMV. One line showed high resistance to inoculations of two WSMV strains. This line had milder symptoms and lower virus titer than control plants after inoculation. After infection, new growth did not show symptoms. The observed resistance was similar to the 'recovery' type resistance described previously using WSMV NIb transgene and in other systems. This line looked morphologically normal but had an unusually high transgene copy number (approximately 90 copies per 2C homozygous genome). Northern hybridization analysis indicated a high level of degraded CP mRNA expression. However, no coat protein expression was detected.
通过生物枪法将小麦线条花叶病毒（WSMV）的外壳蛋白（CP）基因稳定转化到小麦（Triticum aestivum）植株中。获得了11个独立转化的株系，并对其中5个进行了基因表达和对WSMV抗性的分析。一个株系对两种WSMV株系的接种表现出高抗性。接种后，该株系的症状比对照植株轻，病毒滴度也更低。感染后，新长出的部分未表现出症状。观察到的抗性与先前使用WSMV NIb转基因及其他系统所描述的“恢复”型抗性相似。该株系形态上看起来正常，但转基因拷贝数异常高（每2C纯合基因组约90个拷贝）。Northern杂交分析表明CP mRNA表达水平较高且有降解。然而，未检测到外壳蛋白表达。
