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腺病毒核心可作为体外DNA复制的模板。

Adenovirus cores can function as templates in in vitro DNA replication.

作者信息

Goding C R, Russell W C

机构信息

Division of Virology, National Institute for Medical Research, London, UK.

出版信息

EMBO J. 1983;2(3):339-44. doi: 10.1002/j.1460-2075.1983.tb01428.x.

DOI:10.1002/j.1460-2075.1983.tb01428.x
PMID:11894947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC555138/
Abstract

Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein-dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA-protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.

摘要

通过在脱氧胆酸盐存在下于56℃加热温和裂解病毒制备的腺病毒核心，能够在体外DNA复制系统中作为模板发挥作用，既允许通过末端蛋白-dCMP复合物的形成所表明的起始，也允许超过300个核苷酸的延伸。使用核心和DNA-蛋白质复合物作为模板，还证明了DNA旋转酶抑制剂新生霉素通过阻止起始复合物的形成来抑制体外DNA复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c227/555138/e2e7f931513d/emboj00256-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c227/555138/7963370b6f21/emboj00256-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c227/555138/e2e7f931513d/emboj00256-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c227/555138/7963370b6f21/emboj00256-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c227/555138/e2e7f931513d/emboj00256-0049-a.jpg

相似文献

1
Adenovirus cores can function as templates in in vitro DNA replication.腺病毒核心可作为体外DNA复制的模板。
EMBO J. 1983;2(3):339-44. doi: 10.1002/j.1460-2075.1983.tb01428.x.
2
Effects of novobiocin on adenovirus DNA synthesis and encapsidation.新生霉素对腺病毒DNA合成和衣壳化的影响。
Nucleic Acids Res. 1980 Apr 11;8(7):1625-41. doi: 10.1093/nar/8.7.1625.
3
Adenovirus subviral particles and cores can support limited DNA replication.
J Gen Virol. 1989 Dec;70 ( Pt 12):3235-48. doi: 10.1099/0022-1317-70-12-3235.
4
Adenovirus DNA replication in a reconstituted system.腺病毒DNA在重组系统中的复制。
Methods Enzymol. 1995;262:548-60. doi: 10.1016/0076-6879(95)62044-3.
5
Temperature-sensitive initiation and elongation of adenovirus DNA replication in vitro with nuclear extracts from H5ts36-, H5ts149-, and H5ts125-infected HeLa cells.利用来自感染H5ts36、H5ts149和H5ts125的HeLa细胞的核提取物，在体外对腺病毒DNA复制进行温度敏感起始和延伸。
J Virol. 1983 May;46(2):642-8. doi: 10.1128/JVI.46.2.642-648.1983.
6
Role of preterminal protein processing in adenovirus replication.腺病毒复制过程中末端前蛋白加工的作用。
J Virol. 1997 Sep;71(9):6381-9. doi: 10.1128/JVI.71.9.6381-6389.1997.
7
Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.腺病毒DNA的体外复制：一种刺激末端前蛋白-dCMP复合物合成的宿主因子的鉴定。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438-42. doi: 10.1073/pnas.79.21.6438.
8
Initiation rate of adenovirus DNA synthesis in infected cell.感染细胞中腺病毒DNA合成的起始速率。
Biochim Biophys Acta. 1984 May 15;782(1):67-75. doi: 10.1016/0167-4781(84)90107-6.
9
DNA sequences required for the in vitro replication of adenovirus DNA.腺病毒DNA体外复制所需的DNA序列。
Proc Natl Acad Sci U S A. 1984 May;81(10):3069-73. doi: 10.1073/pnas.81.10.3069.
10
Involvement of topoisomerases in replication, transcription, and packaging of the linear adenovirus genome.拓扑异构酶参与线性腺病毒基因组的复制、转录和包装。
J Virol. 1990 Feb;64(2):691-9. doi: 10.1128/JVI.64.2.691-699.1990.

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Assembly and remodeling of viral DNA and RNA replicons regulated by cellular molecular chaperones.由细胞分子伴侣调控的病毒DNA和RNA复制子的组装与重塑。
Biophys Rev. 2018 Apr;10(2):445-452. doi: 10.1007/s12551-017-0333-z. Epub 2017 Nov 22.
2
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本文引用的文献

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Identification of the gene and mRNA for the adenovirus terminal protein precursor.腺病毒末端蛋白前体的基因和信使核糖核酸的鉴定。
Cell. 1981 Feb;23(2):497-508. doi: 10.1016/0092-8674(81)90145-8.
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Effects of novobiocin on adenovirus DNA synthesis and encapsidation.新生霉素对腺病毒DNA合成和衣壳化的影响。
Nucleic Acids Res. 1980 Apr 11;8(7):1625-41. doi: 10.1093/nar/8.7.1625.
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The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets.人类腺病毒12型（Ad12）和腺病毒5型（Ad5）的2.2 kb E1b信使核糖核酸（mRNA）编码两种起始于不同甲硫氨酸密码子（AUG三联体）的肿瘤抗原。
pRNA与噬菌体phi29连接蛋白N端14个氨基酸的结合。
Nucleic Acids Res. 2005 May 10;33(8):2640-9. doi: 10.1093/nar/gki554. Print 2005.
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Requirement of the adenovirus IVa2 protein for virus assembly.腺病毒IVa2蛋白在病毒组装中的需求。
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Role of preterminal protein processing in adenovirus replication.腺病毒复制过程中末端前蛋白加工的作用。
J Virol. 1997 Sep;71(9):6381-9. doi: 10.1128/JVI.71.9.6381-6389.1997.
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Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.NFI与腺病毒复制起点处的腺病毒DNA结合蛋白之间的协同相互作用。
EMBO J. 1989 Jun;8(6):1841-8. doi: 10.1002/j.1460-2075.1989.tb03579.x.
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Recognition mechanisms in the synthesis of animal virus DNA.动物病毒DNA合成中的识别机制。
Biochem J. 1989 Feb 15;258(1):3-16. doi: 10.1042/bj2580003.
Cell. 1981 Nov;27(1 Pt 2):121-31. doi: 10.1016/0092-8674(81)90366-4.
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Nalidixic acid, oxolinic acid, and novobiocin inhibit yeast glycyl- and leucyl-transfer RNA synthetases.萘啶酸、恶喹酸和新生霉素可抑制酵母甘氨酰 - 和亮氨酰 - 转移RNA合成酶。
Science. 1981 Jul 24;213(4506):455-6. doi: 10.1126/science.7017932.
5
Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication.腺病毒末端蛋白前体与其相关DNA聚合酶的分离：两种蛋白在腺病毒DNA复制起始中的作用
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5225-9. doi: 10.1073/pnas.79.17.5225.
6
Function of adenovirus terminal protein in the initiation of DNA replication.腺病毒末端蛋白在DNA复制起始中的作用。
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2221-5. doi: 10.1073/pnas.79.7.2221.
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Adenovirus DNA replication in vitro: purification of the terminal protein in a functional form.腺病毒DNA的体外复制：功能性末端蛋白的纯化
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6779-83. doi: 10.1073/pnas.78.11.6779.
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Formation of a covalent complex between the 80,000-dalton adenovirus terminal protein and 5'-dCMP in vitro.80,000道尔顿腺病毒末端蛋白与5'-脱氧胞苷酸在体外形成共价复合物。
Proc Natl Acad Sci U S A. 1981 May;78(5):2678-82. doi: 10.1073/pnas.78.5.2678.
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Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.腺病毒DNA的体外复制：与新生DNA链共价连接的一种蛋白质的特性
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5105-9. doi: 10.1073/pnas.77.9.5105.
10
Initiation of adenovirus DNA replication: detection of covalent complexes between nucleotide and the 80-kilodalton terminal protein.腺病毒DNA复制的起始：核苷酸与80千道尔顿末端蛋白之间共价复合物的检测
J Virol. 1982 Jan;41(1):265-70. doi: 10.1128/JVI.41.1.265-270.1982.