Llorente M T, Martos A, Castaño A
Division of Environmental Toxicology, CISA-INIA, Madrid, Spain.
Ecotoxicology. 2002 Feb;11(1):27-34. doi: 10.1023/a:1013741012993.
A field study was conducted to investigate the appearance of alterations in the peripheral blood cells of wild populations of fish. Two aspects were evaluated: the appearance of cytogenetic effects, measured as increases on micronuclei frequencies, and the appearance of haematological effects by checking changes in the relative proportion of the different blood cell types. For this purpose common carp (Cyprinus carpio) were caught from four areas along a Spanish river. Three areas were located under the influence of chemical industries and/or a nuclear power plant. The fourth was a clean reference area. Flow cytometry was used to quantify the appearance of micronuclei on the same day of sampling and also after two and twelve months. The alterations in the relative proportion of cell types were counted in blood smears stained with Giemsa. Increases in micronuclei frequencies were observed in fish living in supposedly polluted areas. Alterations of the relative proportions of blood cells were manifested as an increase in white blood cells and as a decrease in red blood cells vs. control area. Since accidental spills have not been reported over this period of time, the alterations observed could suggest that fish are suffering chronic effects due to low level contamination associated with the sampled areas.
开展了一项实地研究，以调查野生鱼类种群外周血细胞的变化情况。评估了两个方面：细胞遗传学效应的表现，以微核频率的增加来衡量；血液学效应的表现，通过检查不同血细胞类型相对比例的变化来确定。为此，从西班牙一条河流的四个区域捕获了鲤鱼（Cyprinus carpio）。其中三个区域受化工行业和/或核电站的影响。第四个区域是清洁的参照区域。在采样当天以及两个月和十二个月后，使用流式细胞术对微核的出现情况进行定量。在用吉姆萨染色的血涂片上计数细胞类型相对比例的变化。在生活在疑似污染区域的鱼类中观察到微核频率增加。血细胞相对比例的变化表现为白细胞增加，与对照区域相比红细胞减少。由于在此期间未报告意外泄漏情况，观察到的变化可能表明鱼类正因与采样区域相关的低水平污染而受到慢性影响。
