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使用抗血清琼脂平板对脑膜炎球菌进行血清分组。

Use of antiserum agar plates for serogrouping of meningococci.

作者信息

Sivonen A, Renkonen O V, Robbins J B

出版信息

J Clin Pathol. 1977 Sep;30(9):834-7. doi: 10.1136/jcp.30.9.834.

DOI:10.1136/jcp.30.9.834
PMID:410847
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC476570/
Abstract

A convenient and reliable method for serogrouping meningococci, based on immunospecific precipitation haloes in antiserum-agar plates, is described. It gave concordant results with conventional slide-agglutination in 900 strains of groups A, B or C tested. The antiserum-agar can also be used as a primary isolation medium for detecting nasopharyngeal carriers of a certain serogroup if antibiotics are added.

摘要

本文描述了一种基于抗血清琼脂平板上的免疫特异性沉淀晕圈对脑膜炎球菌进行血清分组的便捷可靠方法。在对900株A、B或C群菌株进行测试时,该方法与传统玻片凝集法结果一致。如果添加抗生素,抗血清琼脂也可用作检测特定血清群鼻咽部带菌者的初代分离培养基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd6/476570/7361a6f59367/jclinpath00167-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd6/476570/7361a6f59367/jclinpath00167-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd6/476570/7361a6f59367/jclinpath00167-0050-a.jpg

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本文引用的文献

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Serological typing of meningococci by means of micro-precipitation.通过微量沉淀法对脑膜炎球菌进行血清学分型。
Antonie Van Leeuwenhoek. 1961;27:305-15. doi: 10.1007/BF02538460.
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Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides.人类对脑膜炎球菌的免疫力。3. A 群、B 群和 C 群脑膜炎球菌多糖的制备及免疫化学性质。
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Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis.
利用抗血清琼脂检测金黄色葡萄球菌中的荚膜形成。
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Bacterial meningitis in Egypt: analysis of CSF isolates from hospital patients in Cairo, 1977-78.埃及的细菌性脑膜炎:对1977 - 1978年开罗医院患者脑脊液分离株的分析。
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Identity between polysaccharide antigens of Moraxella nonliquefaciens, group B Neisseria meningitidis, and Escherichia coli K1 (non-O acetylated).不动杆菌、B群脑膜炎奈瑟菌和大肠杆菌K1(非O-乙酰化)多糖抗原之间的一致性。
Infect Immun. 1991 Feb;59(2):732-6. doi: 10.1128/iai.59.2.732-736.1991.
8
Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.大肠杆菌K1的形态变异:由荚膜多糖的O-乙酰化作用决定。
J Exp Med. 1979 Mar 1;149(3):669-85. doi: 10.1084/jem.149.3.669.
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J Clin Microbiol. 1979 Oct;10(4):404-8. doi: 10.1128/jcm.10.4.404-408.1979.
10
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J Clin Microbiol. 1979 Sep;10(3):302-7. doi: 10.1128/jcm.10.3.302-307.1979.
改良的用于培养淋病奈瑟菌和脑膜炎奈瑟菌的培养基。
Public Health Rep (1896). 1966 Jun;81(6):559-62.
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Field evaluation of Thayer-Martin medium for identification of meningococcus carriers.
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An Escherichia coli antigen cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b: occurrence among known serotypes, and immunochemical and biologic properties of E. coli antisera toward H. influenzae type b.一种与b型流感嗜血杆菌荚膜多糖发生交叉反应的大肠杆菌抗原:在已知血清型中的出现情况,以及大肠杆菌抗血清针对b型流感嗜血杆菌的免疫化学和生物学特性。
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Bacterial antigens cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b.与b型流感嗜血杆菌荚膜多糖交叉反应的细菌抗原。
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Use of antiserum agar for detection of Haemophilus influenzae type b in the pharynx.使用抗血清琼脂检测咽部的b型流感嗜血杆菌。
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Vaccination against meningococcal group A disease in Finland 1974-75.1974 - 1975年芬兰针对A群脑膜炎球菌疾病的疫苗接种情况。
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Lancet. 1975 Nov 8;2(7941):883-6. doi: 10.1016/s0140-6736(75)92125-x.