Xu Jingwu, Ahmad Ali, Menezes José
Laboratory of Immunovirology, Department of Microbiology and Immunology, University of Montreal and Ste-Justine Hospital, Montreal, Quebec, Canada H3T 1C5.
J Virol. 2002 Apr;76(8):4080-6. doi: 10.1128/jvi.76.8.4080-4086.2002.
The Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP-1) is thought to play a role in the EBV-induced B-cell transformation and immortalization. EBV has also been implicated in certain human T-cell lymphomas; however, the phenotypic effects of the expression of this oncoprotein in T cells are not known. To learn whether LMP-1 also induces phenotypic changes in T cells, we stably expressed it in human cell lines of T and B lineages and 25 LMP-1-expressing T-cell clones and 7 B-cell clones were examined. Our results show for the first time that, in sharp contrast to B cells, LMP-1 preferentially localizes to nuclei in T cells and does not induce the phenotypic changes in these cells that it induces in B cells, does not associate with TRAF proteins, and does not arrest the cell cycle in the G2/M phase. A computer-assisted analysis revealed that LMP-1 lacks the canonical nuclear localization signal. Our results suggest that this oncoprotein may not play the same role in the lymphomagenesis of T cells as it does in B cells.
爱泼斯坦-巴尔病毒（EBV）编码的潜伏膜蛋白1（LMP-1）被认为在EBV诱导的B细胞转化和永生化过程中发挥作用。EBV也与某些人类T细胞淋巴瘤有关；然而，这种癌蛋白在T细胞中表达的表型效应尚不清楚。为了了解LMP-1是否也会诱导T细胞发生表型变化，我们在T和B谱系的人类细胞系中稳定表达了LMP-1，并检测了25个表达LMP-1的T细胞克隆和7个B细胞克隆。我们的结果首次表明，与B细胞形成鲜明对比的是，LMP-1在T细胞中优先定位于细胞核，并且不会在这些细胞中诱导其在B细胞中所诱导的表型变化，不与TRAF蛋白结合，也不会使细胞周期停滞在G2/M期。计算机辅助分析显示LMP-1缺乏典型的核定位信号。我们的结果表明，这种癌蛋白在T细胞淋巴瘤发生过程中可能不像在B细胞中那样发挥相同的作用。
