Dimmer Kai Stefan, Fritz Stefan, Fuchs Florian, Messerschmitt Marlies, Weinbach Nadja, Neupert Walter, Westermann Benedikt
Institut für Physiologische Chemie der Universität München, D-81377 München, Germany.
Mol Biol Cell. 2002 Mar;13(3):847-53. doi: 10.1091/mbc.01-12-0588.
The understanding of the processes underlying organellar function and inheritance requires the identification and characterization of the molecular components involved. We pursued a genomic approach to define the complements of genes required for respiratory growth and inheritance of mitochondria with normal morphology in yeast. With the systematic screening of a deletion mutant library covering the nonessential genes of Saccharomyces cerevisiae the numbers of genes known to be required for respiratory function and establishment of wild-type-like mitochondrial structure have been more than doubled. In addition to the identification of novel components, the systematic screen revealed unprecedented mitochondrial phenotypes that have never been observed by conventional screens. These data provide a comprehensive picture of the cellular processes and molecular components required for mitochondrial function and structure in a simple eukaryotic cell.
要理解细胞器功能和遗传背后的过程，需要鉴定和表征所涉及的分子成分。我们采用基因组学方法来确定酵母中呼吸生长以及具有正常形态的线粒体遗传所需的基因互补情况。通过系统筛选覆盖酿酒酵母非必需基因的缺失突变体文库，已知对呼吸功能和建立野生型样线粒体结构所必需的基因数量增加了一倍多。除了鉴定新成分外，系统筛选还揭示了传统筛选从未观察到的前所未有的线粒体表型。这些数据提供了一个简单真核细胞中线粒体功能和结构所需的细胞过程和分子成分的全面图景。
