Hajnická Valéria, Kost'álová Daniela, Svecová Danka, Sochorová Ruzena, Fuchsberger Norbert, Tóth Jaroslav
Planta Med. 2002 Mar;68(3):266-8. doi: 10.1055/s-2002-23126.
The effect of the crude extract and of two alkaloid fractions prepared from Mahonia aquifolium on interleukin-8 (IL-8) production in the lipopolysaccharide (LPS)-stimulated human monocytic cell line THP-1 was studied. The production of IL-8 by cells stimulated with 20 ng/ml LPS after 48 h treatment with 20 microg/ml crude extract was inhibited by about 30 %. LPS-stimulated cells treated with 0.1 microg/ml bisbenzylisoquinoline alkaloid fraction exhibited a 40% inhibition of IL-8 production in comparison with control cells. The fraction of protoberberine alkaloids had no significant inhibitory activity. Weak or no inhibition of IL-8 production was found after treatment with 0.5 microg/ml of protoberberine alkaloids berberine and jatrorrhizine and with berbamine from the group of BBI alkaloids. In contrast, the production was inhibited after treatment with BBI alkaloids baluchistine (about 20%) and aromoline (up to 30%).
研究了阔叶十大功劳粗提物及从中制备的两种生物碱组分对脂多糖（LPS）刺激的人单核细胞系THP-1中白细胞介素-8（IL-8）产生的影响。用20μg/ml粗提物处理48小时后，用20ng/ml LPS刺激的细胞产生的IL-8受到约30%的抑制。与对照细胞相比，用0.1μg/ml双苄基异喹啉生物碱组分处理的LPS刺激细胞的IL-8产生受到40%的抑制。原小檗碱生物碱组分没有显著的抑制活性。用0.5μg/ml的原小檗碱生物碱黄连素、药根碱以及来自双苄基异喹啉生物碱组的小檗胺处理后，发现对IL-8产生的抑制作用较弱或无抑制作用。相比之下，用双苄基异喹啉生物碱巴鲁奇斯廷（约20%）和阿罗莫林（高达30%）处理后，IL-8的产生受到抑制。
