Gođevac Dejan, Damjanović Ana, Stanojković Tatjana P, Anđelković Boban, Zdunić Gordana
Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia.
J Pharm Biomed Anal. 2018 Feb 20;150:9-14. doi: 10.1016/j.jpba.2017.11.075. Epub 2017 Dec 1.
Herein, we propose a H NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of H and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberinе and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
在此，我们提出一种基于氢核磁共振（¹H NMR）的代谢组学方法，以揭示阔叶十大功劳茎皮中的细胞毒性代谢物。通过对¹H和二维核磁共振谱进行全面分析，无需事先分离，即可鉴定阔叶十大功劳提取物中的初级和次级代谢物。采用正交偏最小二乘法（OPLS）多元分析方法，将植物提取物的化学成分与针对人宫颈腺癌细胞系的细胞毒性活性结果进行关联。原小檗碱生物碱黄连素和巴马汀，以及双苄基异喹啉生物碱小檗胺被确定为OPLS模型中最具影响力的成分，具有最高的细胞毒性活性。
