Carrasco L, Núñez A, Salguero F J, Díaz San Segundo F, Sánchez-Cordón P, Gómez-Villamandos J C, Sierra M A
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
J Comp Pathol. 2002 Feb-Apr;126(2-3):194-201. doi: 10.1053/jcpa.2001.0543.
To determine, in the acute form of African swine fever (ASF), the relationship between the appearance of pulmonary oedema and viral replication and expression of cytokines by pulmonary intravascular macrophages (PIMs), 14 pigs were inoculated intramuscularly with ASF virus (strain España'70) and killed in pairs on days 1-7 post-inoculation. Samples of lung were examined immunohistochemically and ultrastructurally. The immunohistochemical study was carried out with antibodies against interleukin-1 alpha (IL-1alpha), tumour necrosis factor-alpha (TNF-alpha), viral antigen of ASF (Vp73) and a myeloid marker (SWC3). Viral replication was observed mainly in PIMs, which at the same time showed intense activation, accompanied by the expression of IL-1alpha and TNF-alpha. The occurrence of interstitial oedema, neutrophil sequestration and fibrin microthrombi in septal capillaries coincided with high degrees of cytokine expression by infected PIMs. Alveolar macrophages did not show a significant change in cytokine expression as a result of ASF infection, and viral replication was detected in only a low percentage of these cells.
为了确定在非洲猪瘟（ASF）急性型中，肺水肿的出现与肺部血管内巨噬细胞（PIMs）的病毒复制及细胞因子表达之间的关系，14头猪经肌肉接种ASF病毒（西班牙'70株），并在接种后第1至7天成对处死。对肺组织样本进行免疫组织化学和超微结构检查。免疫组织化学研究使用了抗白细胞介素-1α（IL-1α）、肿瘤坏死因子-α（TNF-α）、ASF病毒抗原（Vp73）和一种髓系标志物（SWC3）的抗体。病毒复制主要在PIMs中观察到，同时这些细胞表现出强烈激活，并伴有IL-1α和TNF-α的表达。间质水肿、中性粒细胞滞留和间隔毛细血管中的纤维蛋白微血栓的出现与受感染PIMs高度的细胞因子表达相一致。肺泡巨噬细胞因ASF感染在细胞因子表达上未显示出显著变化，并且仅在这些细胞的低比例中检测到病毒复制。
