从猪、人和大鼠表皮分离出的细胞的脂质组成。

Lipid compositions of cells isolated from pig, human, and rat epidermis.

作者信息

Gray G M, Yardley H J

出版信息

J Lipid Res. 1975 Nov;16(6):434-40.

PMID:1194786

Abstract

Epidermal slices from pig, human, and rat skin were treated with dilute buffered trypsin solution (0.005%, w/v), and suspensions of mixed basal and spinous cells were obtained in good yield. Total lipids accounted for approximately 8% of the pig, 10% of the human, and 20% of the rat epidermal cell (dry weight). Phospholipids in pig, human, and rat cells accounted for, respectively, 62%, 53%, and 35% of the total lipids. Phosphatidylcholine (34-38%), phosphatidylethanolamine (18-23%), and sphingomyelin (17-21%) were major compounds in all species. The major neutral lipids were sterols (mostly cholesterol) and triglycerides. Free fatty acids were a major lipid class in pig and human cells, whereas wax esters were a major component in rat epidermal cells. Nearly half (45%) of the sterols in rat cells but less than 10% of those in pig and human cells were esterified. Cholest-7-ene-3beta-ol accounted for 20% of the total sterols in rat cells. Cholesteryl sulfate and ceramide were minor lipids in the three species. The predominant glycosphingolipid (greater than 99%) was glucosylceramide, which accounted for 7% and 9%, respectively, of the total lipids in pig and human cells. A significant proportion (pig, 17%; human, 11%) of the fatty acids in the glucosylceramides were C26:0 and C28:0.

摘要

用稀释的缓冲胰蛋白酶溶液（0.005%，w/v）处理猪、人及大鼠皮肤的表皮切片，可高产率获得基底细胞和棘细胞的混合悬浮液。总脂质分别约占猪表皮细胞（干重）的8%、人表皮细胞的10%和大鼠表皮细胞的20%。猪、人及大鼠细胞中的磷脂分别占总脂质的62%、53%和35%。磷脂酰胆碱（34 - 38%）、磷脂酰乙醇胺（18 - 23%）和鞘磷脂（17 - 21%）是所有物种中的主要化合物。主要的中性脂质是固醇（主要是胆固醇）和甘油三酯。游离脂肪酸是猪和人细胞中的主要脂质类别，而蜡酯是大鼠表皮细胞中的主要成分。大鼠细胞中近一半（45%）的固醇被酯化，而猪和人细胞中被酯化的固醇不到10%。胆甾 - 7 - 烯 - 3β - 醇占大鼠细胞总固醇的20%。硫酸胆固醇和神经酰胺是这三个物种中的次要脂质。主要的糖鞘脂（>99%）是葡糖神经酰胺（glucosylceramide），分别占猪和人细胞总脂质的7%和9%。葡糖神经酰胺中相当一部分脂肪酸（猪为17%；人为11%）是C26:0和C28:0。