Gray G M, Yardley H J
J Lipid Res. 1975 Nov;16(6):441-7.
Preparations representing populations of (a) basal and spinous cells, (b) granular cells, and (c) stratum corneum cells were obtained by successive treatments of epidermal slices from pig skin with dilute buffered trypsin solutions. Total lipids accounted for about 8% of the cell dry weight in each of the three populations. Phospholipids, which predominated in the basal and spinous cells, accounted for only 21% of the total lipids in the granular cells and less than 0.1% in the stratum corneum. The latter cells contained more cholesterol (23% of total lipid) than either the granular cells (18%) or the basal and spinous cells (8%). The proportion of ceramide was also much higher in the stratum corneum (17%) and granular cells (9%) than in the basal and spinous cells (1%). The relative amounts of glycosphingolipid (glucosylceramide) and cholesteryl sulfate in the total lipids of stratum corneum cells were less than half those in the granular cells and basal and spinous cells. A novel phospholipid was a major component (26% of total) of the phospholipids from granular cells. The compound, which was partially characterized, contained phosphorus, fatty acids, and glycerol (molar ratio 1:3:2) and appeared to be a neutral derivative of phosphatidic acid.
通过用稀释的缓冲胰蛋白酶溶液对猪皮肤的表皮切片进行连续处理，获得了代表（a）基底细胞和棘细胞、（b）颗粒细胞以及（c）角质层细胞群体的制剂。在这三个细胞群体中，总脂质约占细胞干重的8%。在基底细胞和棘细胞中占主导地位的磷脂，在颗粒细胞中仅占总脂质的21%，在角质层中则不到0.1%。角质层细胞含有的胆固醇（占总脂质的23%）比颗粒细胞（18%）或基底细胞和棘细胞（8%）都多。神经酰胺的比例在角质层（17%）和颗粒细胞（9%）中也比在基底细胞和棘细胞（1%）中高得多。角质层细胞总脂质中糖鞘脂（葡糖神经酰胺）和硫酸胆固醇的相对含量不到颗粒细胞以及基底细胞和棘细胞中的一半。一种新型磷脂是颗粒细胞磷脂的主要成分（占总量的26%）。该化合物经部分表征，含有磷、脂肪酸和甘油（摩尔比为1:3:2），似乎是磷脂酸的一种中性衍生物。
