Fukai K, Maeda Y, Fujimoto K, Itou T, Sakai T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, 252-8510, Kanagawa, Japan.
Vet Microbiol. 2002 May 24;86(4):343-9. doi: 10.1016/s0378-1135(02)00023-8.
G8 bovine group A rotavirus was the most predominant serotype in calf diarrheal fecal specimens examined between 1995 and 1996 in Japan [Vet. Microbiol. 66 (1999) 301]. To date, no evidence that G8 was the most predominant in the typeable specimens has been observed outside Japan. To investigate whether G8 continues to be as common as G6 and G10, the incidence of the main serotypes was determined in the same area (Kagoshima prefecture) between 1997 and 1998 by reverse transcription-polymerase chain reaction. From a total of 104 rotavirus-positive specimens, we successfully identified G type in 79 (76.0%) and P type in 71 (68.3%). The combination of G and P types varied periodically; in 1995, G10P[11] was most common. The most predominant types changed drastically in 1996, and G8 and mixed P types were the most predominant. A dramatic shift of the most predominant type occurred again in 1997 when G6P[5] was most common. This frequency of G6P[5] also continued in 1998. These results suggest that the serotypes prevailing in certain areas change periodically.
G8型牛A组轮状病毒是1995年至1996年在日本检测的犊牛腹泻粪便样本中最主要的血清型[《兽医微生物学》66(1999)301]。迄今为止，在日本以外地区尚未观察到G8型在可分型样本中最为主要的证据。为了调查G8型是否继续与G6型和G10型一样常见，1997年至1998年期间，通过逆转录-聚合酶链反应在同一地区（鹿儿岛县）测定了主要血清型的发病率。在总共104份轮状病毒阳性样本中，我们成功鉴定出79份(76.0%)的G型和71份(68.3%)的P型。G型和P型的组合呈周期性变化；1995年，G10P[11]最为常见。1996年，最主要的类型发生了急剧变化，G8型和混合P型最为主要。1997年，最主要的类型再次发生显著转变，G6P[5]最为常见。1998年，G6P[5]的这种频率也持续存在。这些结果表明，某些地区流行的血清型会周期性变化。
