Demirbilek S, Aydin G, Yücesan S, Vural H, Bitiren M
Department of Pediatric Surgery, Harran University Faculty of Medicine, Sanliurfa, Turkey.
Eur J Pediatr Surg. 2002 Feb;12(1):8-12. doi: 10.1055/s-2002-25082.
Esophageal strictures are characterized by excess deposition of collagen in the esophageal wall. Polyunsaturated phosphatidyl-choline (PPC) stimulates collagen breakdown in experimental models of liver cirrhosis and colitis. This study was done in order to investigate the therapeutical effect of PPC in preventing esophageal strictures due to alkali-induced esophageal burns in rats. Fifty-five albino rats were divided into four groups as follows: control group (Group A, 10 rats), rats with sham operation and treated with saline (Group B, 15 rats), rats with esophageal burns only (Group C, 15 rats), and PPC-fed rats with esophageal burns (Group D, 15 rats). A standard esophageal burn was produced as described by Gehanno. PPC was administered orally to Group D rats in doses of 100 mg/day for four weeks. All animals were sacrificed on the 28th day of the experiment. Hydroxyproline levels in esophageal tissue was determined in each rat, and histopathologic evaluation was performed for each group. Hydroxyproline levels were significantly lower in the PPC-fed rats than in the rats with pure esophageal burns (p < 0.001). Histopathologically, collagen deposition in the submucosa and tunica muscularis was lower in Group D rats (PPC-fed rats with esophageal burn) than Group C rats (pure esophageal burn) (p < 0.05). As a result of our study, we concluded that PPC has an ameliorating effect on stricture formation after alkali-induced corrosive esophageal burns in rats.
食管狭窄的特征是食管壁中胶原蛋白过度沉积。多不饱和磷脂酰胆碱（PPC）在肝硬化和结肠炎的实验模型中可刺激胶原蛋白分解。本研究旨在探讨PPC对预防大鼠碱液所致食管烧伤后食管狭窄的治疗效果。55只白化大鼠分为四组如下：对照组（A组，10只大鼠）、假手术并给予生理盐水治疗的大鼠（B组，15只大鼠）、仅食管烧伤的大鼠（C组，15只大鼠）以及给予PPC的食管烧伤大鼠（D组，15只大鼠）。按照Gehanno所述方法造成标准食管烧伤。D组大鼠口服PPC，剂量为100mg/天，持续四周。在实验第28天处死所有动物。测定每只大鼠食管组织中的羟脯氨酸水平，并对每组进行组织病理学评估。给予PPC的大鼠的羟脯氨酸水平显著低于单纯食管烧伤的大鼠（p<0.001）。组织病理学上，D组大鼠（给予PPC的食管烧伤大鼠）黏膜下层和肌层的胶原蛋白沉积低于C组大鼠（单纯食管烧伤大鼠）（p<0.05）。作为我们研究的结果，我们得出结论，PPC对大鼠碱液所致腐蚀性食管烧伤后狭窄形成具有改善作用。
