Pallier Coralie, Tebourbi Lamia, Chopineau-Proust Stéphanie, Schoevaert Damien, Nordmann Patrice, Testart Jacques, Courtot Anne-Marie
Service de Bactériologie-Virologie-Parasitologie-Hygiène, CHU Bicêtre, Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre, , France.
Hum Reprod. 2002 May;17(5):1281-7. doi: 10.1093/humrep/17.5.1281.
The effect of viral particles on the motility of human sperm and the relationship between sperm and virus are of importance particularly in assisted fertilization.
We incubated ejaculated sperm with or without seminal fluid with either herpes simplex virus type 2 (HSV2) or human cytomegalovirus (HCMV). For each experiment, 5 x 10(5) sperm were incubated with a viral load of between 10(4) and 10(6) plaque-forming units.
We detected no apparent variations in the percentage of motile forms when sperm were incubated with either HSV2 or HCMV. Using a computer-aided semen analysis system, a slight difference was reported in the percentage of motile forms when seminal fluid-free sperm were incubated with HSV2 (57.18 versus 64.43 in the control). Although the mean amplitude of lateral head displacement and the curvilinear velocity were significantly higher in infected sperm, the difference in straight line velocity was not statistically significantly different. Few viral particles (HSV2 or HCMV) adhered to the sperm membrane in the presence of seminal fluid. However, more particles stuck when in the absence of seminal fluid, particularly with HSV2 (8% of sperm sections for HSV2; 4% for HCMV).
The relationship between sperm and viruses depends on the type of virus present as well as the presence or absence of seminal fluid. Motility is not a good enough criterion on which to prove the presence of viral elements, either in the medium or on the sperm.
病毒颗粒对人类精子活力的影响以及精子与病毒之间的关系尤为重要，特别是在辅助生殖方面。
我们将射出的精子与含有或不含精液的单纯疱疹病毒2型（HSV2）或人巨细胞病毒（HCMV）一起孵育。对于每个实验，将5×10⁵个精子与病毒载量在10⁴至10⁶个空斑形成单位之间进行孵育。
当精子与HSV2或HCMV孵育时，我们未检测到活动形式百分比的明显变化。使用计算机辅助精液分析系统，当无精液的精子与HSV2孵育时，活动形式百分比报告有轻微差异（对照组为57.18%对64.43%）。虽然感染精子的平均头部侧向位移幅度和曲线速度显著更高，但直线速度的差异无统计学意义。在有精液存在时，很少有病毒颗粒（HSV2或HCMV）附着在精子膜上。然而，在无精液时，更多颗粒附着，特别是HSV2（HSV2为8%的精子切片；HCMV为4%）。
精子与病毒之间的关系取决于存在的病毒类型以及精液的有无。活力不是证明培养基或精子中存在病毒成分的足够好的标准。
