González Luis Miguel, Montero Estrella, Puente Sabino, López-Velez Rogelio, Hernández Marisela, Sciutto Edda, Harrison Leslie J S, Parkhouse R Michael E, Gárate Teresa
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
Diagn Microbiol Infect Dis. 2002 Apr;42(4):243-9. doi: 10.1016/s0732-8893(01)00356-x.
The potential value of PCRs in the species-specific diagnosis of have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin.
利用来自不同地理区域的牛带绦虫和猪带绦虫样本，研究了聚合酶链反应（PCR）在物种特异性诊断中的潜在价值。检测种间差异的PCR基于牛带绦虫/猪带绦虫特异性的HDP2 DNA片段序列以及核糖体DNA内部转录间隔区1和间隔区2（ITS-1和ITS-2）的序列。对DNA分离株的这种PCR分析证实了形态学诊断，并且能够对因太小或破碎而无法进行形态学鉴定的样本进行物种鉴定，牛带绦虫（22个）和猪带绦虫（3个）地理分离株之间存在清晰且一致的种间差异。通过对PCR扩增产物的分析（PCR-RFLP），同样研究了这些物种内可能存在的种内基因组变异性，仅在肯尼亚的一株牛带绦虫分离株中发现了例外情况，该分离株产生了独特的PCR-RFLP模式，不同于墨西哥（1个样本）和西班牙（7个样本）来源的牛带绦虫DNA。
