Bornscheuer Uwe T
Institute for Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr. 16, Greifswald, Germany.
FEMS Microbiol Rev. 2002 Mar;26(1):73-81. doi: 10.1111/j.1574-6976.2002.tb00599.x.
Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of ester bonds and are widely distributed in animals, plants and microorganisms. Beside lipases, a considerable number of microbial carboxyl esterases have also been discovered and overexpressed. This review summarizes their properties and classification. Special emphasis is given on their application in organic synthesis for the resolution of racemates and prostereogenic compounds. In addition, recent results for altering their properties by directed evolution are presented.
酯酶（EC 3.1.1.x）是一类多样的水解酶，催化酯键的裂解和形成，广泛分布于动物、植物和微生物中。除脂肪酶外，还发现并大量表达了许多微生物羧基酯酶。本文综述了它们的性质和分类。特别强调了它们在有机合成中用于拆分外消旋体和前手性化合物的应用。此外，还介绍了通过定向进化改变其性质的最新研究成果。
