Holland Roberto, de Souza Valdir, Nery Mauro Juvenal, Bernabé o Felicio Estrada, Filho José Arlindo Otoboni, Junior Eloi Dezan, Murata Sueli Satomi
Department of Endodontics, Faculdade de Odontologia de Araçatuba, UNESP, São Paulo, Brazil.
J Endod. 2002 Mar;28(3):173-6. doi: 10.1097/00004770-200203000-00007.
This study was conducted to observe the rat subcutaneous connective tissue reaction to implanted dentin tubes that were filled with mineral trioxide aggregate, Sealapex, Calciobiotic Root Canal Sealer (CRCS), Sealer 26, and the experimental material, Sealer Plus. The animals were sacrificed after 7 and 30 days, and the specimens were prepared for histological analysis after serial sections with a hard-tissue microtome. The undecalcified sections were examined with polarized light after staining according to the Von Kossa technique for calcium. At the tube openings, there were Von Kossa-positive granules that were birefringent to polarized light. Next to these granulations, there was irregular tissue, like a bridge, that was Von Kossa-positive. The dentin walls of the tubes exhibited a structure highly birefringent to polarized light, usually like a layer, in the tubules. These results were observed with all the studied materials, except the CRCS, which didn't exhibit any kind of mineralized structure. The results suggest that among the materials studied, the CRCS could have the least possibility of encouraging hard tissue deposition.
本研究旨在观察大鼠皮下结缔组织对植入的牙本质小管的反应，这些牙本质小管填充有三氧化矿物凝聚体、Sealapex、钙生物根管封闭剂（CRCS）、封闭剂26以及实验材料Sealer Plus。在7天和30天后处死动物，用硬组织切片机进行连续切片后制备标本用于组织学分析。根据冯·科萨钙染色技术对未脱钙切片进行偏振光检查。在小管开口处，有对偏振光双折射的冯·科萨阳性颗粒。在这些颗粒旁边，有不规则的组织，类似桥状，呈冯·科萨阳性。小管的牙本质壁在小管中呈现出对偏振光高度双折射的结构，通常像一层。除CRCS外，所有研究材料均观察到这些结果，CRCS未表现出任何矿化结构。结果表明，在所研究的材料中，CRCS促进硬组织沉积的可能性最小。
