Fahey John V, Wira Charles R
Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, New Hampshire 03756, USA.
J Infect Dis. 2002 Jun 1;185(11):1606-13. doi: 10.1086/340512. Epub 2002 May 17.
The objective of this study was to examine the production of antibacterial factor(s) by uterine epithelial cells from pre- and postmenopausal women. Apical rinses from polarized epithelial cells recovered from women at the proliferative and secretory stages of the menstrual cycle were equally effective in killing Staphylococcus aureus and Escherichia coli, but those from postmenopausal women were not. Secretory leukocyte protease inhibitor (SLPI) concentrations of apical washes from premenopausal women were significantly higher than those obtained from postmenopausal women. SLPI production correlated with bactericidal activity with respect to menstrual status and time in culture. Anti-SLPI antibody significantly decreased bactericidal activity of premenopausal epithelial cell rinses. The endometrial epithelial cell line HEC-1A did not have a bactericidal effect, nor did it produce SLPI. In contrast, HEC-1B cells produced SLPI and a factor that inhibited bacterial growth. These results indicate that menstrual status (pre- vs. postmenopausal) influences the production of SLPI and bactericidal activity by uterine epithelial cells.
本研究的目的是检测绝经前和绝经后女性子宫上皮细胞抗菌因子的产生情况。从处于月经周期增殖期和分泌期的女性中回收的极化上皮细胞的顶端冲洗液在杀死金黄色葡萄球菌和大肠杆菌方面同样有效，但绝经后女性的冲洗液则不然。绝经前女性顶端冲洗液中分泌型白细胞蛋白酶抑制剂（SLPI）的浓度显著高于绝经后女性。SLPI的产生与月经状态和培养时间的杀菌活性相关。抗SLPI抗体显著降低了绝经前上皮细胞冲洗液的杀菌活性。子宫内膜上皮细胞系HEC-1A没有杀菌作用，也不产生SLPI。相反，HEC-1B细胞产生SLPI和一种抑制细菌生长的因子。这些结果表明，月经状态（绝经前与绝经后）会影响子宫上皮细胞SLPI的产生和杀菌活性。
