Kashefi Kazem, Tor Jason M, Holmes Dawn E, Gaw Van Praagh Catherine V, Reysenbach Anna-Louise, Lovley Derek R
Int J Syst Evol Microbiol. 2002 May;52(Pt 3):719-728. doi: 10.1099/00207713-52-3-719.
A novel, regular to irregular, coccoid-shaped, anaerobic, Fe(III)-reducing microorganism was isolated from the Guaymas Basin hydrothermal system at a depth of 2000 m. Isolation was carried out with a new technique using Fe(III) oxide as the electron acceptor for the recovery of colonies on solid medium. The isolate, designated strain 234T, was strictly anaerobic and exhibited a tumbling motility. The cells had a single flagellum. Strain 234T grew at temperatures between 65 and 90 degrees C, with an optimum at about 88 degrees C. The optimal salt concentration for growth was around 19 g l(-1). The isolate was capable of growth with H2 as the sole electron donor coupled to the reduction of Fe(III) without the need for an organic carbon source. This is the first example of a dissimilatory Fe(III)-reducing micro-organism capable of growing autotrophically on hydrogen. In addition to molecular hydrogen, strain 234T oxidizes pyruvate, acetate, malate, succinate, peptone, formate, fumarate, yeast extract, glycerol, isoleucine, arginine, serine, glutamine, asparagine, stearate, palmitate, valerate, butyrate and propionate with the reduction of Fe(III). This isolate is the first example of a hyperthermophile capable of oxidizing long-chain fatty acids anaerobically. Isolate 234T grew exclusively with Fe(III) as the sole electron acceptor. The G+C content was 58.7 mol%. Based on detailed analysis of its 16S rDNA sequence, G+C content, distinguishing physiological features and metabolism, strain 234T is proposed to represent a novel genus within the Archaeoglobales. The name proposed for strain 234T is Geoglobus ahangari gen. nov., sp. nov..
从瓜伊马斯海盆2000米深处的热液系统中分离出一种新型、规则至不规则、球状、厌氧、能还原Fe(III)的微生物。采用一种新技术进行分离，该技术以氧化铁作为电子受体，用于在固体培养基上回收菌落。分离出的菌株命名为234T，严格厌氧，具有翻滚运动性。细胞有一根单鞭毛。菌株234T在65至90摄氏度之间生长，最适温度约为88摄氏度。生长的最适盐浓度约为19 g l(-1)。该分离株能够以H2作为唯一电子供体生长，并与Fe(III)的还原偶联，无需有机碳源。这是第一例能够以氢气为自养生长的异化Fe(III)还原微生物。除分子氢外，菌株234T还能氧化丙酮酸、乙酸、苹果酸、琥珀酸、蛋白胨、甲酸、富马酸、酵母提取物、甘油、异亮氨酸、精氨酸、丝氨酸、谷氨酰胺、天冬酰胺、硬脂酸、棕榈酸、戊酸、丁酸和丙酸，并还原Fe(III)。该分离株是第一例能够厌氧氧化长链脂肪酸的嗜热菌。分离株234T仅以Fe(III)作为唯一电子受体生长。G+C含量为58.7 mol%。基于对其16S rDNA序列、G+C含量、独特生理特征和代谢的详细分析，菌株234T被提议代表古球菌目内的一个新属。为菌株234T提议的名称是阿氏地球球菌（Geoglobus ahangari），新属，新种。
