Satomi M, Kimura B, Hayashi M, Shouzen Y, Okuzumi M, Fujii T
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1341-8. doi: 10.1099/00207713-48-4-1341.
Two strains of helical, halophilic, Gram-negative, heterotrophic bacteria were isolated from kusaya gravy which is a traditional Japanese fermented brine. These strains were motile by means of a single polar or bipolar tuft flagellum. They had a large cell size, were helical, formed coccoid bodies, were microaerophilic and had quinone type Q-8. The DNA G + C content of the strains was 44-45 mol %. A detailed investigation of the phenotypic, chemotaxonomic and phylogenetic characteristics of the strains revealed that they represent a new species of halophilic helical bacteria. The sequence of the 16S rRNA gene of strain H7T, designated the type strain of the new isolates, and all of the Oceanospirillum species except for Oceanospirillum linum were determined. Phylogenetic analysis indicated that these strains were closely related to Oceanospirillum minutulum, with enough distance to separate the O. minutulum/new isolate H7T cluster from Oceanospirillum sensu stricto on the genus level. It is proposed that a new genus, Marinospirillum, be created; this genus should include Marinospirillum minutulum ATCC 19193T (formerly Oceanospirillum minutulum) as the type species, as well as Marinospirillum megaterium JCM 10129T (= H7T).
从日本传统发酵卤汁粕渍汁中分离出两株螺旋形、嗜盐、革兰氏阴性、异养细菌。这些菌株借助单根极鞭毛或双极丛鞭毛运动。它们细胞体积大，呈螺旋形，形成球状菌体，为微需氧菌，醌型为Q-8。这些菌株的DNA G + C含量为44 - 45摩尔%。对这些菌株的表型、化学分类和系统发育特征进行的详细研究表明，它们代表了嗜盐螺旋菌的一个新物种。测定了新分离株的模式菌株H7T以及除亚麻海洋螺菌外所有海洋螺菌属物种的16S rRNA基因序列。系统发育分析表明，这些菌株与微小海洋螺菌密切相关，在属水平上与狭义海洋螺菌属有足够的距离将微小海洋螺菌/新分离株H7T聚类区分开来。建议创建一个新属，即海螺旋菌属；该属应包括微小海螺旋菌ATCC 19193T（原名微小海洋螺菌）作为模式种，以及巨大海螺旋菌JCM 10129T（= H7T）。
