Ordal G W, Gibson K J
J Bacteriol. 1977 Jan;129(1):151-5. doi: 10.1128/jb.129.1.151-155.1977.
Conditions for assaying chemotaxis in Bacillus subtilis are described. The chemotaxis medium we used afforded excellent motility for hours. In it, chemotaxis measured by capillary assays was insensitive to pH between 5.5 and 9, and to temperature between 28 degrees C and 42 degrees C. Chemotaxis was observed toward all 20 common amino acids, with thresholds varying from 3nM for alanine to 0.1 mM for glutamate, in the capillary assay, and from 0.1 muM for alanine to 0.32 mM for glutamate in the microscope assay.
本文描述了枯草芽孢杆菌趋化性测定的条件。我们使用的趋化性培养基能在数小时内提供良好的运动性。在该培养基中,通过毛细管测定法测得的趋化性在pH值5.5至9之间以及温度28摄氏度至42摄氏度之间不敏感。在毛细管测定法中,观察到对所有20种常见氨基酸都有趋化性,阈值从丙氨酸的3nM到谷氨酸的0.1mM不等,而在显微镜测定法中,丙氨酸的阈值为0.1μM,谷氨酸为0.32mM。