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枯草芽孢杆菌趋化排斥物的识别位点。

Recognition sites for chemotactic repellents of Bacillus subtilis.

作者信息

Ordal G W

出版信息

J Bacteriol. 1976 Apr;126(1):72-9. doi: 10.1128/jb.126.1.72-79.1976.

Abstract

Repellents of Bacillus subtilis include many membrane-active compounds, such as uncouplers of oxidative phosphorylation, local anesthetics, chlorpromazine (a central nervous system depressant), and tetraphenylboron (a lipophilic anion). Normally, bacteria swim smoothly, and occasionally tumble, but addition of repellent causes all bacteria to tumble, then later resume original frequency of swimming and tumbling (adaptation). Bacteria adapted to repellent can then be tested to determine the minimum concentration (threshold) of the same or different repellents that causes tumbling. The results indicate that repellents act at (saturable) recognition sites, which differ for chemically different species. An implication is that uncouplers of oxidative phosphorylation affect cell properties by interaction at specific locations.

摘要

枯草芽孢杆菌的驱避剂包括许多膜活性化合物,如氧化磷酸化解偶联剂、局部麻醉剂、氯丙嗪(一种中枢神经系统抑制剂)和四苯基硼(一种亲脂性阴离子)。正常情况下,细菌平稳游动,偶尔翻滚,但添加驱避剂会使所有细菌翻滚,随后恢复原来的游动和翻滚频率(适应)。然后可以对适应了驱避剂的细菌进行测试,以确定导致翻滚的相同或不同驱避剂的最低浓度(阈值)。结果表明,驱避剂作用于(可饱和的)识别位点,不同化学种类的识别位点不同。这意味着氧化磷酸化解偶联剂通过在特定位置的相互作用来影响细胞特性。

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本文引用的文献

1
A new class of uncoupling agents--carbonyl cyanide phenylhydrazones.一类新型解偶联剂——羰基氰苯腙。
Biochem Biophys Res Commun. 1962 May 4;7:272-5. doi: 10.1016/0006-291x(62)90189-4.
2
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation.氧化磷酸化和光合磷酸化中的化学渗透偶联
Biol Rev Camb Philos Soc. 1966 Aug;41(3):445-502. doi: 10.1111/j.1469-185x.1966.tb01501.x.
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Negative chemotaxis in Escherichia coli.大肠杆菌中的负趋化性。
J Bacteriol. 1974 May;118(2):560-76. doi: 10.1128/jb.118.2.560-576.1974.
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The gradient-sensing mechanism in bacterial chemotaxis.细菌趋化作用中的梯度感应机制。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509-12. doi: 10.1073/pnas.69.9.2509.
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Conservation and transformation of energy by bacterial membranes.细菌膜对能量的守恒与转化
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