Pogue Gregory P, Lindbo John A, Garger Stephen J, Fitzmaurice Wayne P
Large Scale Biology Corporation, 3333 Vaca Valley Pkwy, Vacaville, CA 95688, USA.
Annu Rev Phytopathol. 2002;40:45-74. doi: 10.1146/annurev.phyto.40.021102.150133. Epub 2002 Feb 20.
Historically, the study of plant viruses has contributed greatly to the elucidation of eukaryotic biology. Recently, concurrent with the development of viruses into expression vectors, the biotechnology industry has developed an increasing number of disease therapies utilizing recombinant proteins. Plant virus vectors are viewed as a viable option for recombinant protein production. Employing pathogens in the process of creating added value to agriculture is, in effect, making an ally from an enemy. This review discusses the development and use of viruses as expression vectors, with special emphasis on (+) strand RNA virus systems. Further, the use of virus expression vectors in large-scale agricultural settings to produce recombinant proteins is described, and the technical challenges that need to be addressed by agriculturists and molecular virologists to fully realize the potential of this latest evolution of plant science are outlined.
从历史上看，植物病毒的研究对阐明真核生物生物学做出了巨大贡献。最近，随着病毒发展成为表达载体，生物技术产业开发了越来越多利用重组蛋白的疾病疗法。植物病毒载体被视为生产重组蛋白的可行选择。在农业创造附加值的过程中利用病原体，实际上是在化敌为友。本综述讨论了病毒作为表达载体的开发和使用，特别强调了（+）链RNA病毒系统。此外，还描述了在大规模农业环境中使用病毒表达载体生产重组蛋白的情况，并概述了农学家和分子病毒学家为充分实现植物科学这一最新进展的潜力而需要应对的技术挑战。
