苏云金芽孢杆菌中高水平β-外毒素I与cry1B存在的相关性。
Correspondence of high levels of beta-exotoxin I and the presence of cry1B in Bacillus thuringiensis.
作者信息
Espinasse Sylvain, Gohar Michel, Chaufaux Josette, Buisson Christophe, Perchat Stéphane, Sanchis Vincent
机构信息
Unité de Recherches de Lutte Biologique, INRA La Minière, 78285 Guyancourt, France.
出版信息
Appl Environ Microbiol. 2002 Sep;68(9):4182-6. doi: 10.1128/AEM.68.9.4182-4186.2002.
Abstract
Examination of 640 natural isolates of Bacillus thuringiensis showed that the 58 strains (9%) whose supernatants were toxic to Anthonomus grandis (Coleoptera: Curculionidae) produced between 10 and 175 micro g of beta-exotoxin I per ml. We also found that 55 (46%) of a sample of 118 strains whose culture supernatants were not toxic to A. grandis nevertheless produced between 2 and 5 micro g/ml. However, these amounts of beta-exotoxin I were below the threshold for detectable toxicity against this insect species. Secretion of large amounts of beta-exotoxin I was strongly associated with the presence of cry1B and vip2 genes in the 640 natural B. thuringiensis isolates studied. We concluded that strains carrying cry1B and vip2 genes also possess, on the same plasmid, genetic determinants necessary to promote high levels of production of beta-exotoxin I.
摘要
对640株苏云金芽孢杆菌自然分离株的检测表明，其培养上清液对大谷象（鞘翅目：象甲科）有毒性的58株菌株（9%），每毫升产生10至175微克的β-外毒素I。我们还发现，在118株培养上清液对大谷象无毒的菌株样本中，有55株（46%）每毫升仍产生2至5微克。然而，这些β-外毒素I的量低于对该昆虫物种可检测到毒性的阈值。在所研究的640株苏云金芽孢杆菌自然分离株中，大量β-外毒素I的分泌与cry1B和vip2基因的存在密切相关。我们得出结论，携带cry1B和vip2基因的菌株在同一质粒上还拥有促进β-外毒素I高水平产生所必需的遗传决定因素。
相似文献
1
Correspondence of high levels of beta-exotoxin I and the presence of cry1B in Bacillus thuringiensis.苏云金芽孢杆菌中高水平β-外毒素I与cry1B存在的相关性。
Appl Environ Microbiol. 2002 Sep;68(9):4182-6. doi: 10.1128/AEM.68.9.4182-4186.2002.
2
An ABC transporter from Bacillus thuringiensis is essential for beta-exotoxin I production.来自苏云金芽孢杆菌的一种ABC转运蛋白对于β-外毒素I的产生至关重要。
J Bacteriol. 2002 Nov;184(21):5848-54. doi: 10.1128/JB.184.21.5848-5854.2002.
3
Occurrence and linkage between secreted insecticidal toxins in natural isolates of Bacillus thuringiensis.苏云金芽孢杆菌自然分离株中分泌型杀虫毒素的发生与连锁关系。
Curr Microbiol. 2003 Dec;47(6):501-7. doi: 10.1007/s00284-003-4097-2.
4
Prevalence of beta-exotoxin, diarrhoeal toxin and specific delta-endotoxin in natural isolates of Bacillus thuringiensis.苏云金芽孢杆菌自然分离株中β-外毒素、腹泻毒素和特定δ-内毒素的流行情况。
FEMS Microbiol Lett. 1998 Mar 1;160(1):55-60. doi: 10.1111/j.1574-6968.1998.tb12890.x.
5
Correlation between serovars of Bacillus thuringiensis and type I beta-exotoxin production.苏云金芽孢杆菌血清型与I型β-外毒素产生之间的相关性。
J Invertebr Pathol. 2003 Jan;82(1):57-62. doi: 10.1016/s0022-2011(02)00199-4.
6
Recombinant strain of Bacillus thuringiensis producing Cyt1A, Cry11B, and the Bacillus sphaericus binary toxin.产生Cyt1A、Cry11B和球形芽孢杆菌二元毒素的苏云金芽孢杆菌重组菌株。
Appl Environ Microbiol. 2003 Feb;69(2):1331-4. doi: 10.1128/AEM.69.2.1331-1334.2003.
7
Lack of toxicity to adults of the Mexican fruit fly (Diptera: Tephritidae) of beta-exotoxin in Bacillus thuringiensis endotoxin preparations.
J Econ Entomol. 2000 Aug;93(4):1076-9. doi: 10.1603/0022-0493-93.4.1076.
8
Screening and characterization of Bacillus thuringiensis isolates from Brazil for the presence of coleoptera-specific cry genes.对来自巴西的苏云金芽孢杆菌分离株进行鞘翅目特异性cry基因存在情况的筛选与鉴定。
Microbiol Res. 2000 Mar;154(4):355-62. doi: 10.1016/S0944-5013(00)80010-5.
9
An extracytoplasmic-function sigma factor is involved in a pathway controlling beta-exotoxin I production in Bacillus thuringiensis subsp. thuringiensis strain 407-1.一种胞外功能sigma因子参与了苏云金芽孢杆菌苏云金亚种407-1菌株中控制β-外毒素I产生的途径。
J Bacteriol. 2004 May;186(10):3108-16. doi: 10.1128/JB.186.10.3108-3116.2004.
10
PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains.基于聚合酶链反应（PCR）预测苏云金芽孢杆菌菌株中I型β-外毒素的产生
J Invertebr Pathol. 2014 Oct;122:28-31. doi: 10.1016/j.jip.2014.08.001. Epub 2014 Aug 15.
引用本文的文献
1
Repertoire of the Virulence Factors Unrelated to Major Classes of Protein Toxins and Its Role in Specificity of Host-Pathogen Interactions.非主要蛋白毒素类毒力因子库及其在宿主-病原体相互作用特异性中的作用。
Toxins (Basel). 2019 Jun 17;11(6):347. doi: 10.3390/toxins11060347.
2
Isolation and characterization of strains native to Assam soil of North East India.印度东北部阿萨姆邦土壤中本土菌株的分离与特性研究
3 Biotech. 2017 Oct;7(5):303. doi: 10.1007/s13205-017-0935-y. Epub 2017 Sep 9.
3
Thuringiensin: a thermostable secondary metabolite from Bacillus thuringiensis with insecticidal activity against a wide range of insects.苏云金菌素：一种来自苏云金芽孢杆菌的热稳定次生代谢物，对多种昆虫具有杀虫活性。
Toxins (Basel). 2014 Jul 25;6(8):2229-38. doi: 10.3390/toxins6082229.
4
Identification of distinct Bacillus thuringiensis 4A4 nematicidal factors using the model nematodes Pristionchus pacificus and Caenorhabditis elegans.利用模式线虫秀丽隐杆线虫和太平洋小杆线虫鉴定苏云金芽孢杆菌4A4的不同杀线虫因子。
Toxins (Basel). 2014 Jul 14;6(7):2050-63. doi: 10.3390/toxins6072050.
5
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.为生物防治产品开发而需要同时评估苏云金芽孢杆菌菌株的晶体和上清液相关的杀虫毒性。
Toxins (Basel). 2014 May 20;6(5):1598-614. doi: 10.3390/toxins6051598.
6
Molecular characterization and genetic diversity of insecticidal crystal protein genes in native Bacillus thuringiensis isolates.本土苏云金芽孢杆菌分离株杀虫晶体蛋白基因的分子特征和遗传多样性。
Curr Microbiol. 2013 Apr;66(4):323-30. doi: 10.1007/s00284-012-0273-6. Epub 2012 Dec 4.
7
An extracytoplasmic-function sigma factor is involved in a pathway controlling beta-exotoxin I production in Bacillus thuringiensis subsp. thuringiensis strain 407-1.一种胞外功能sigma因子参与了苏云金芽孢杆菌苏云金亚种407-1菌株中控制β-外毒素I产生的途径。
J Bacteriol. 2004 May;186(10):3108-16. doi: 10.1128/JB.186.10.3108-3116.2004.
本文引用的文献
1
Sample preparation for beta-exotoxin determination in Bacillus thuringiensis cultures by reversed-phase high-performance liquid chromatography.
Anal Biochem. 2001 Nov 1;298(1):112-7. doi: 10.1006/abio.2001.5373.
2
Update on the detection of beta-exotoxin in Bacillus thuringiensis strains by HPLC analysis.通过高效液相色谱分析检测苏云金芽孢杆菌菌株中β-外毒素的最新进展。
J Appl Microbiol. 2001 Apr;90(4):643-7. doi: 10.1046/j.1365-2672.2001.01288.x.
3
How Bacillus thuringiensis has evolved specific toxins to colonize the insect world.苏云金芽孢杆菌是如何进化出特定毒素以在昆虫世界中繁衍的。
Trends Genet. 2001 Apr;17(4):193-9. doi: 10.1016/s0168-9525(01)02237-5.
4
Bacillus thuringiensis and its pesticidal crystal proteins.苏云金芽孢杆菌及其杀虫晶体蛋白。
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
5
Prevalence of beta-exotoxin, diarrhoeal toxin and specific delta-endotoxin in natural isolates of Bacillus thuringiensis.苏云金芽孢杆菌自然分离株中β-外毒素、腹泻毒素和特定δ-内毒素的流行情况。
FEMS Microbiol Lett. 1998 Mar 1;160(1):55-60. doi: 10.1111/j.1574-6968.1998.tb12890.x.
6
Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects.Vip3A，一种新型的苏云金芽孢杆菌营养期杀虫蛋白，对鳞翅目昆虫具有广泛的活性。
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5389-94. doi: 10.1073/pnas.93.11.5389.
7
Cloning of a cryV-type insecticidal protein gene from Bacillus thuringiensis: the cryV-encoded protein is expressed early in stationary phase.苏云金芽孢杆菌cryV型杀虫蛋白基因的克隆：cryV编码蛋白在稳定期早期表达。
J Bacteriol. 1996 Apr;178(7):2141-4. doi: 10.1128/jb.178.7.2141-2144.1996.
8
9
PCR analysis of the cryI insecticidal crystal family genes from Bacillus thuringiensis.苏云金芽孢杆菌cryI杀虫晶体蛋白家族基因的聚合酶链反应（PCR）分析
Appl Environ Microbiol. 1994 Jan;60(1):353-6. doi: 10.1128/aem.60.1.353-356.1994.
10
Generalized transduction in Bacillus thuringiensis var. berliner 1715 using bacteriophage CP-54Ber.使用噬菌体CP-54Ber对苏云金芽孢杆菌柏林变种1715进行广义转导
J Gen Microbiol. 1980 Nov;121(1):203-12. doi: 10.1099/00221287-121-1-203.
Zotero 插件