Eick Sigrun, Pfister Wolfgang
Department of Microbiological Microbiology, University Hospital of Jena, Germany.
J Clin Periodontol. 2002 Jul;29(7):638-44. doi: 10.1034/j.1600-051x.2002.290708.x.
Microbiological laboratory procedures are involved in diagnosis and therapy control of progressive and refractory forms of periodontitis. In recent years techniques have been developed based on the detection of nucleic acids. The purpose of this study was to validate the commercially available micro-Dent(R) test which employs probes for A. actinomycetemcomitans, P. gingivalis, P. intermedia, B. forsythus and T. denticola.
122 plaque samples obtained from periodontal pockets with various depths from 33 early onset periodontitis (EOP) patients and 15 periodontally healthy subjects were analysed by cultivation and the microDent(R) kit.
Both cultivation and the nucleic acid based assay showed a positive correlation of pocket depth with the frequency and quantity of periodontopathogenic species. T. denticola was found only in pockets > 4 mm in EOP patients. Comparison of the two methods revealed that the microDent(R) kit identified both P. gingivalis and B. forsythus more often than did the cultivation method.
Nucleic acid techniques should replace cultivation methods as gold standard in microbiological diagnosis of progressive periodontitis. The micro-Dent(R) kit can be recommended for microbiological laboratories analysing subgingival plaque samples.
微生物实验室程序参与了牙周炎进展期和难治性形式的诊断和治疗控制。近年来，基于核酸检测开发了一些技术。本研究的目的是验证市售的micro-Dent(R)检测，该检测采用针对伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、福赛坦氏菌和齿垢密螺旋体的探针。
通过培养和microDent(R)试剂盒对从33例早发性牙周炎（EOP）患者和15名牙周健康受试者的不同深度牙周袋中获取的122份菌斑样本进行分析。
培养和基于核酸的检测均显示牙周袋深度与牙周致病菌种的频率和数量呈正相关。在EOP患者中，仅在深度>4mm的牙周袋中发现了齿垢密螺旋体。两种方法的比较显示，microDent(R)试剂盒比培养法更常检测到牙龈卟啉单胞菌和福赛坦氏菌。
在进展性牙周炎的微生物诊断中，核酸技术应取代培养方法作为金标准。micro-Dent(R)试剂盒可推荐给分析龈下菌斑样本的微生物实验室。
