Yong Dongeun, Lee Kyungwon, Yum Jong Hwa, Shin Hee Bong, Rossolini Gian Maria, Chong Yunsop
Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemoon-ku, Seoul 120-752, Korea.
J Clin Microbiol. 2002 Oct;40(10):3798-801. doi: 10.1128/JCM.40.10.3798-3801.2002.
Rapid detection of metallo-beta-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 micro g of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20 degrees C, respectively.
快速检测产金属β-内酰胺酶(MBL)的革兰氏阴性杆菌对于防止其传播至关重要。使用含亚胺培南加750微克乙二胺四乙酸(EDTA)的纸片法能区分所有产MBL的假单胞菌,对不动杆菌的敏感性和特异性分别为95.7%和91.0%。亚胺培南-EDTA纸片在4℃和-20℃分别可稳定保存12周和16周。