伊拉克济加尔省产碳青霉烯酶临床分离株的表型和基因型特征

Phenotypic and genotypic characterization of carbapenemase-producing clinical isolates in Thi-Qar, Iraq.

作者信息

Khalaf Moslim Mohsin, Abd Al-Mayahi Firas Srhan

机构信息

Department of Pathological Analysis, College of Applied Medical Sciences, Al-Shatrah University, Thi-Qar, Iraq.

Department of Biology, College of Science, University of Al-Qadisiya, Al-Qadisiya, Iraq.

出版信息

Iran J Microbiol. 2025 Apr;17(2):268-277. doi: 10.18502/ijm.v17i2.18387.

BACKGROUND AND OBJECTIVES

The emergence of carbapenem resistance in poses an urgent threat. The study aims to assess carbapenem resistance and the presence of carbapenemase genes in clinical isolates from Thi-Qar Hospital, Iraq.

MATERIALS AND METHODS

A total of 2203 specimens were collected from patients at two hospitals between January and October 2024. was identified via biochemical tests and confirmed with the Vitek2® system. Antibiotic sensitivity was evaluated using disc diffusion, and carbapenemase production was investigated through combined disc tests (CDT) and modified Hodge tests (MHT). PCR was used to detect carbapenemase genes.

RESULTS

Out of 2203 specimens, 1212 (55.02%) exhibited bacterial growth, with accounting for 15.35% (186/1212) of isolates. Among these, 40 (21.51%) were resistant to at least one carbapenem. CDT identified 10, and MHT identified 1 as a carbapenemase producer. The most detected gene was (60.00%), followed by (40.00%) and (15.00%). and were found in 5.00% of isolates each. No , , , , , , or was detected.

CONCLUSION

The high prevalence of carbapenem resistance and the corresponding encoding genes in in Thi-Qar province pose a concerning challenge for managing serious infections caused by this pathogen.

背景与目的

碳青霉烯耐药性的出现构成了紧迫威胁。本研究旨在评估伊拉克济加尔省医院临床分离株中的碳青霉烯耐药性及碳青霉烯酶基因的存在情况。

材料与方法

2024年1月至10月期间，从两家医院的患者中总共收集了2203份标本。通过生化试验进行鉴定，并使用Vitek2®系统进行确认。采用纸片扩散法评估抗生素敏感性，通过联合纸片试验（CDT）和改良 Hodge试验（MHT）研究碳青霉烯酶的产生情况。使用聚合酶链反应（PCR）检测碳青霉烯酶基因。

结果

在2203份标本中，1212份（55.02%）呈现细菌生长，其中[具体细菌名称]占分离株的15.35%（186/1212）。在这些菌株中，40份（21.51%）对至少一种碳青霉烯耐药。CDT鉴定出10份，MHT鉴定出1份为碳青霉烯酶产生菌。检测到最多的基因是[具体基因名称1]（60.00%），其次是[具体基因名称2]（40.00%）和[具体基因名称3]（15.00%）。[具体基因名称4]和[具体基因名称5]在各5.00%的分离株中被发现。未检测到[其他具体基因名称]。