采用AMPLICOR HIV-1 MONITOR检测试剂盒1.5版（超敏）测定1型人类免疫缺陷病毒RNA在脑脊液中的稳定性。
Stability of human immunodeficiency virus type 1 RNA in cerebrospinal fluid determined with the AMPLICOR HIV-1 MONITOR test, version 1.5 (ultrasensitive).
作者信息
Singer Elyse J, Aronow H Aaron, Lee Shing-Yi, Hinkin Charles H, Lazarus Tadd
机构信息
Department of Neurology, School of Medicine, University of California at Los Angeles, 11645 Wilshire Boulevard, Suite 770, Los Angeles, CA 90025, USA.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3863-4. doi: 10.1128/JCM.40.10.3863-3864.2002.
Abstract
We evaluated the effects of time, temperature, freezing, and thawing on the cerebrospinal fluid viral load by using the Roche AMPLICOR HIV-1 MONITOR test, version 1.5 (ultrasensitive). While a statistically significant decrease from the baseline was observed at 24 h, but not at 6 or 12 h, and with one freeze-thaw cycle, all changes were within the range of intra-assay variability.
摘要
我们使用罗氏AMPLICOR HIV-1监测测试1.5版（超灵敏）评估了时间、温度、冷冻和解冻对脑脊液病毒载量的影响。虽然在24小时时观察到与基线相比有统计学意义的下降，但在6小时或12小时时未观察到，并且经过一个冻融循环后，所有变化都在分析内变异范围内。
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本文引用的文献
1
Cerebrospinal fluid viral load is related to cortical atrophy and not to intracerebral calcifications in children with symptomatic HIV disease.脑脊液病毒载量与有症状的HIV疾病儿童的皮质萎缩有关，而与脑内钙化无关。
J Neurovirol. 2000 Oct;6(5):390-7. doi: 10.3109/13550280009018303.
2
Quantitation of human immunodeficiency virus type 1 RNA in different biological compartments.1型人类免疫缺陷病毒RNA在不同生物区室中的定量分析。
J Clin Microbiol. 2000 Apr;38(4):1414-8. doi: 10.1128/JCM.38.4.1414-1418.2000.
3
Cerebrospinal fluid HIV RNA originates from both local CNS and systemic sources.脑脊液中的HIV RNA来源于局部中枢神经系统和全身各处。
Neurology. 2000 Feb 22;54(4):927-36. doi: 10.1212/wnl.54.4.927.
4
The contribution of assay variation and biological variation to the total variability of plasma HIV-1 RNA measurements. The Women Infant Transmission Study Clinics. Virology Quality Assurance Program.检测变异和生物学变异对血浆HIV-1 RNA测量总变异性的贡献。母婴传播研究诊所。病毒学质量保证项目。
AIDS. 1999 Nov 12;13(16):2269-79. doi: 10.1097/00002030-199911120-00009.
5
Plasma and cerebrospinal fluid human immunodeficiency virus type-1 (HIV-1) RNA levels in HIV-related cognitive impairment.与HIV相关的认知障碍中血浆和脑脊液1型人类免疫缺陷病毒（HIV-1）RNA水平
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6
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7
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10
Optimization of specimen-handling procedures for accurate quantitation of levels of human immunodeficiency virus RNA in plasma by reverse transcriptase PCR.通过逆转录聚合酶链反应优化血浆中人类免疫缺陷病毒RNA水平准确定量的样本处理程序。
J Clin Microbiol. 1998 Apr;36(4):1070-3. doi: 10.1128/JCM.36.4.1070-1073.1998.
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